基于差异凝胶双向电泳技术的冠心病不稳定性心绞痛血瘀证患者血浆差异蛋白特征研究

Gel Electrophoresis Analysis on Plasma Differential Protein in Patients with Unstable Angina of Blood-stasis Pattern

目的通过对冠心病心绞痛血瘀证患者和健康人血浆的差异凝胶电泳图谱检测,寻找其血浆差异蛋白,探索其蛋白质组学特点。方法采用差异凝胶双向电泳和串联质谱对12例冠心病不稳定性心绞痛气虚血瘀证患者、12例痰瘀互阻证患者和12名健康人去高丰度蛋白血浆进行比较蛋白质组学研究,寻找心绞痛血瘀证血浆差异蛋白。结果初步发现在冠心病不稳定性心绞痛气虚血瘀证和痰瘀互阻证两组患者表达均变化的蛋白有Haptoglobin β chain、DBP、HBB、HBA、Transthyretin、ApoA-Ⅰ、ApoA-Ⅳ。仅在冠心病不稳定性心绞痛痰瘀互阻证表达变化的蛋白有Haptoglobin α1 chain、α-1-acid glycoprotein、ApoC-Ⅲ、ApoA-Ⅱ、ApoC-Ⅱ、ApoJ、Haptoglobin α 2 chain。仅在冠心病不稳定性心绞痛气虚血瘀证表达变化的蛋白α1-antitryp-sin、Fibrinogen γ chain、Fibrin β。结论冠心病不稳定性心绞痛气虚血瘀证与痰瘀互阻证在蛋白质层面的部分共性特征表现为炎症反应、代谢紊乱(包括血脂、血氧等)。

Objective To seek the plasma differential proteins in patients with unstable angina of blood-stasis pattern （UA-BSS） for exploring the proteomic specialty in them by way of two-dimensional difference gel electrophoresis （DIGE） detection on plasma of patients and healthy persons. Methods Using DIGE and tandem mass spectrometry, comparative proteomic study was conducted on the plasma of 12 UA patients of qi-deficiency and blood-stasis pattern （UA-QBS）, 12 UA patients of phlegm-stasis cross-blocking pattern （UA-PSS） and 12 healthy volunteers. Results Preliminary results showed that Haptoglobin β chain, DBP, HBB, HBA, Transthyretin, ApoA-Ⅰ, ApoA-Ⅳ were significantly differentially expressed in both patterns, while Haptoglobin α1 chain, α-1-acid glycoprotein , ApoC-Ⅲ, ApoA-Ⅱ, ApoC-Ⅱ, ApoJ, and Haptoglobin α 2 chain were only seen differentially expressed in the UA-PSS patients, α1-antitrypsin, Fibrinogen γ chain, and Fibrin β were only seen differentially expressed in UA-QBS patients. Conclusion The common proteomics characteristics of patients of QBS and PSS patterns may be correlated with inflammatory reaction and metabolic disturbance （including blood lipid and blood oxygen）.