狼疮3号颗粒治疗系统性红斑狼疮的实验研究

Experimental Study on Treatment of Systemic Lupus Erythematosus with Langchuang-3 Granule

目的通过研究中药狼疮3号颗粒(Langchuang-3 granule,LC-3)对BXSB系统性红斑狼疮(systemic lupus erythematosus,SLE)模型鼠体重、自身抗体、脾指数、胸腺指数以及肾脏免疫复合物沉积的影响,探讨其对SLE的作用机制。方法将BXSBSLE模型鼠随机分成4组:对照组、中药组、西药组、中西药组。采用动态观察法测定小鼠体重变化,重量法测定小鼠脾指数及胸腺指数;双抗体夹心酶联免疫吸附法(ELISA)测定小鼠血清中抗核抗体(ANA)的含量;直接免疫荧光法测定小鼠肾脏中免疫球蛋白G(IgG)、补体C3的含量。结果中药组及中西药组小鼠体重增长明显,与对照组比较,差异有统计学意义(P<0.05,P<0.01);中药组已降低的胸腺指数升高,与空白对照组比较,差异有统计学意义,对已升高的脾指数作用不明显,西药组脾指数及胸腺指数均下调,而中西药组升高的脾指数降低(P<0.01),降低的胸腺指数升高(P<0.05);各给药组自身抗体(ANA)水平均降低,转为阴性(P<0.01);小鼠肾脏IgG、补体C3的荧光强度均降低,与对照组比较,差异有统计学意义(P<0.05,P<0.01)。结论 LC-3与西药类固醇激素均对BXSB狼疮模型鼠有明显的治疗作用,中西药合用疗效优于单独应用中药或西药。

Objective To investigate the mechanism of action of Chinese drug Langchuang-3 Granule （LC-3） in treating systemic lupus erythematosus （SLE） through observing its effects on body weight （BW）, antinuclear antibody （ANA）, spleen index and thymus index in BXSB SLE model mice, as well as the deposition of immune complex in mice′ renal glomeruli. Methods SLE model mice were randomized into 4 groups： the control group （A）, the LC-3 treated group （B）, the Western drug treated group （C）, and the LC-3 combined Western drug treated group （D）. BW of mice was dynamically observed; spleen index （SI） and thymus index （TI） were measured by weighting method; serum anti-ANA level was detected by ELISA; IgG and complement C3 in renal glomeruli were analysed by direct immunofluorescence. Results BW of mice remarkably increased after treatment in Group B and D, showing a significant difference to that in Group A （P〈0.05）. Lower TI level and higher SI level were found in the model mice. As compared with Group A, TI was higher in Group B and D, SI was lower in Group D, and both indices were lower in Group C （P〈0.05 or P〈0.01）. Level of ANA decreased （converting from positive to negative） remarkably in all the three treated groups （P〈0.01）. The fluorescence intensity of IgG and C3 in the renal tissue was weaker in Group B, C （both P〈0.05） and D （P〈0.01） than that in Group A. Conclusion Both LC-3 and Western medicine showed remarkable effects in treating SLE model mice, and the combination of the two could display an effect better than that of using either alone.