苦豆碱对急性期溃疡性结肠炎小鼠的治疗作用

Effects of aloperine on dextran sulfate sodium-induced acute ulcerative colitis in mice

目的:研究苦豆碱(aloperine)对葡聚糖硫酸钠(dextran sulfate sodium,DSS)致小鼠急性期溃疡性结肠炎(ulcerative colitis,UC)的治疗作用及机制。方法:建立DSS诱导的小鼠急性期UC模型,给予苦豆碱(50,25 mg.kg-1)处理,观察各组小鼠的一般状态、疾病活动度指数、结肠组织病理学变化和组织学评分;检测小鼠血清中IL-1β,IL-4和IL-6的含量以及结肠组织核因子(NF-κBp65)的表达水平。结果:苦豆碱能明显改善结肠炎小鼠的一般状况和结肠组织病理学损伤、降低血清IL-1β和IL-6水平、升高IL-4水平、抑制NF-κBp65的表达。结论:苦豆碱能有效地缓解结肠炎小鼠的炎症反应,其机制可能与抑制NF-κBp65表达、调节促炎因子和抗炎因子之间的平衡有关。

Objective：To investigate the therapeutic effects of aloperine on acute ulcerative colitis（UC） induced by dextran sulfate sodium（DSS） in mice,and to explore the possible mechanism.Methods： Acute ulcerative colitis was induced by DSS,and aloperine was given orally for 8 days at the same time.The general situations and histopathological changes were observed during the experiment.Disease activity index（DAI） and histological score were evaluated.The levels of serum IL-1,IL-4 and IL-6 were determined by ELISA.The expression of nuclear factor（NF-κBp65） in colon was detected by immunohistochemistry and Western blotting.Results： Aloperine greatly ameliorated the symptom of UC and histopathological changes as compared with UC control.The DAI and histological score decreased significantly,the levels of serum IL-1β and IL-6 and NF-κBp65 in colon were obviously reduced,and the expression of serum IL-4 increased after aloperine treatment.Conclusion： Aloperine can effectively attenuate DSS-induced acute ulcerative colitis through decreasing NF-κBp65 expression and regulating balance of inflammatory cytokines.