耐力训练对高脂膳食大鼠骨骼肌线粒体脂肪氧化及PGC-1α基因表达的影响

Effects of Endurance Training on Mitochondrial Lipid Oxidation and PGC-1α Gene Expression of High-Fat Diet Rats' Skeletal Muscle

目的:研究耐力训练对高脂膳食大鼠骨骼肌线粒体脂肪氧化相关酶活性及基因表达的影响。方法:40只SD大鼠,随机分成普通膳食对照组(C)与耐力训练组(E);高脂膳食对照组(H)与耐力训练组(R),每组10只。两耐力训练组大鼠进行8周跑台训练。结果:耐力训练使高脂膳食大鼠体重(P=0.000)I、R指数(P=0.021)显著降低并维持在正常水平,使骨骼肌线粒体β-HAD(P=0.011)、CS活性(P=0.047)、CPT-1βmRNA(P=0.037)及PGC-1α蛋白表达水平(P=0.007)显著增高。结论:耐力训练通过适度调节参与线粒体脂肪氧化关键酶β-HAD、CS活性及CPT-1β、PGC-1α基因表达,优化高脂膳食机体在线粒体水平的脂肪氧化能力。

Purpose：Investigate the effects of 8-week endurance training on mitochondrial lipid oxidation-related enzymes and genes expression in high-fat diet rats.Methods：40 male SD rats were randomly assigned to standard diet control group（C）and endurance training group（E）,high-fat diet control group（H）and endurance training group（R）,ten in each group.The two endurance training groups＇ rats were accustomed to 8-week treadmill training.Results：Endurance training efficiently decreased the weight（P=0.000）and IR index（P=0.021）in the high-fat diet rats as well as significantly increased the activity of β-HAD（P=0.011）,CS（P=0.047）and the expression of CPT-1β mRNA（P=0.037）and PGC-1α protein（P=0.007）in high-fat diet rats＇ skeletal muscle mitochondrial.Conclusion：Endurance training optimizes the whole process of fat oxidation at the mitochondrial level through moderate regu-lating the activity of β-HAD and CS enzymes and the expression of CPT-1β and PGC-1α,which are involved in mitochondrial fat oxidation.