还原型谷胱甘肽对毒鼠强中毒大鼠心脑的保护作用

Heart and brain protection of reduced glutathione in acute tetramine poisoning rats

目的:探讨还原型谷胱甘肽(GSH)对毒鼠强(TET)中毒大鼠心脑的保护作用。方法:健康SD大鼠30只,随机分为假手术组、对照组、GSH预处理组,每组10只。通过TET灌胃染毒大鼠制备动物实验模型,染毒前30 min分别给予对照组、GSH预处理组腹腔注射生理盐水及GSH(1 000 mg/kg),观察各组大鼠的心脑组织病理学改变,免疫组织化学方法评价脑组织γ-氨基丁酸(GABA)、神经元特异性烯醇化酶(NSE)的表达水平。结果:GSH能明显延长TET染毒大鼠的惊厥潜伏期、缓解中毒症状、降低中毒大鼠的病死率,增加脑组织GABA的水平、减少NSE释放,减轻染毒大鼠心脑组织损伤。结论:GSH对TET中毒大鼠有保护作用,为进一步的临床使用提供理论依据。

Objective：To investigate the heart and brain protection of reduced glutathione（GSH） in acute tetramine（TET） poisoning rat. Method：Thirty rats were divided randomly and equally into sham-operated,control and GSH-pretreated group（n =10,each group）,the sodium chloride or GSH（1000 mg/kg body wt.） were administered in rats by intraperitoneal injection 30 min before the acute toxicity animal model was performed with TET by intragastric（0.2 mg/kg body wt.）.The changes of cell pathology in the heart and brain were investigated with HE method.The expression of gamma-aminobutyric acid（GABA） and neurone specific enolase（NSE） in brain of rats poisoned by TET was analyzed with immunofluorescecnce method.Result：GSH could obviously delay poisoned rats onset of convulsion,improve poisoned rats symptoms after acute tetramine poisoning,reduce poisoned rats mortality,augmentation expression of GABA,decrease release of NSE in brains,and attenuate myocardial/brain damage of poisoned rats.Conclusion：GSH can effectively antagonize acute toxicity of tetramine in rat,and open a new possibility for further clinical application.