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创伤后应激障碍大鼠蓝斑核神经元细胞凋亡的实验研究 被引量：7

APOPTOSIS IN LOCUS COERULEUS NEURONS OF PTSD-LIKE RATS
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摘要 目的探讨创伤后应激障碍(PTSD)大鼠蓝斑核神经元Caspase-3和Caspase-9的表达变化。方法成年健康雄性Wistar大鼠50只,随机分为连续单一刺激(single prolonged stress,SPS)模型1d、4d、7d、14d组和对照组,应用免疫组化、免疫荧光和RT-PCR方法检测PTSD大鼠蓝斑核神经元Caspase-3和Caspase-9的表达变化。结果SPS刺激后1d,4d,Caspase-9的表达逐渐增强,7d和14d逐渐下降,Caspase-3于SPS刺激后7d表达最多,14d出现下降。结论蓝斑神经元细胞凋亡可能是导致PTSD患者蓝斑功能失调的重要原因之一。 Objective To investigate the expressions of Caspase-3 and Caspase-9 in locus coeruleus of rats with post-traumatic stress disorder （PTSD）. Methods A total of 50 healthy, male Wistar rats were randomly divided into 1, 4, 7 and 14d groups of single prolonged stress （SPS） and a normal control group. Immunohistochemistry, immunofluorescence and RT-PCR were used to observe and detect the changes in the expressions of Caspase-3 and Caspase-9 in the locus coeruleus neurons. Results Caspase-9 expression showed gradual increase on ld and 4d, and gradual decrease on 7d and 14d. Caspase-3 peaked at 7 d and then gradually decreased. Conclusion The apoptosis of locus coeruleus neurons might play an im- portant role in the dysfunction of locus coeruleus in PTSD patients.
作者 李慢 韩芳 石玉秀
机构地区 中国医科大学基础医学院组织学与胚胎学教研室
出处 《中国组织化学与细胞化学杂志》 CAS CSCD 2010年第1期15-19,共5页 Chinese Journal of Histochemistry and Cytochemistry
基金 国家自然科学基金资助项目(30600341) 教育部博士点基金(20092104110016)
关键词 创伤后应激障碍 蓝斑 凋亡 CASPASE-3 CASPASE-9 PTSD Locus coeruleus Apoptosis Caspase-3 Caspase-9
分类号 R332.81 [医药卫生—人体生理学]
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参考文献10

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二级参考文献40

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共引文献27

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中国组织化学与细胞化学杂志
2010年 第1期

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