柿叶提取物对小鼠链脲佐菌素糖尿病的影响

Effects of extracts from persimmon leaves in diabetic mice induced by streptozotocin

目的:观察柿叶各提取物对链脲佐菌素(STZ)所致糖尿病小鼠的血糖、胰岛素及血脂的影响。方法:将实验小鼠随机分为13组:正常对照组,模型组,二甲双胍组,乙醇总提物高、低剂量组,氯仿萃取部位高、低剂量组,乙酸乙酯萃取部位高、低剂量组,正丁醇萃取部位高、低剂量组,水提醇沉部位高、低剂量组。除正常对照组外其它组均以腹腔注射STZ(150mg/kg)建立Ⅱ型糖尿病小鼠模型。连续给药15d后,分别测定各小鼠空腹血糖(FBG)、空腹胰岛素(FINS)和血脂。结果:与模型组比较,乙醇总提物、氯仿萃取部位、乙酸乙酯萃取部位、正丁醇萃取部位和水提醇沉部位对STZ所致糖尿病小鼠有显著降低血糖的作用,降低胰岛素抵抗指数(IR)及甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C),显著提高胰岛素敏感指数(ISI)和高密度脂蛋白胆固醇(HDL-C)。结论:柿叶提取物有降低STZ致糖尿病小鼠模型的血糖水平,改善胰岛素抵抗,调节脂代谢紊乱的作用,其作用有效成分主要存在于氯仿萃取部位、乙酸乙酯萃取部位、正丁醇萃取部位和水提醇沉部位。

Objective：To observe the effects of different extractives from persimmon leaves on glycemia,insulin and lipidemia in diabetic mice induced by streptozotocin（STZ）.Methods：The experimental mice were randomly divided into 13 groups：Control group,Model group,DMBG group,high and low dosage groups of total ethanol fraction,high and low dosage groups of chloroform fraction,high and low dosage groups of ethyl acetate fraction,high and low dosage groups of n-butanol fraction,high and low dosage groups of water-extract and ethanol-precipitate fraction.Except the control group,all groups were established type 2 diabetes mice model by intraperitoneal injection of STZ（150mg/kg）.After the drugs were administrated to experimental mice for 15 days,and the fasting blood glucose（FBG）,insulin（FINS）and blood lipid were determined in diabetic mice.Results：Compared with the Model group,the ethanol fraction,ethylacetate fraction,n-butanol fraction and water extract and alcohol precipitate fraction could significantly decrease blood glucose in STZ-induced diabetic mice,and decrease insulin resistance index（IR）and the contents of TG,TC and LDL-C in the serum and increased significantly insulin sensitivity index（ISI）and HDL-C content in the serum.Conclusion：Extracts from persimmon leaves can significantly reduce fasting blood glucose level in diabetic mice induced by STZ,improve insulin resistance and adjust metabolic disturbance.Chloroform fraction,ethyl acetate fraction,n-butanol fraction and water-extract and ethanol-precipitate fraction are main effective composition of effect.