摘要
以白桦花药(单核靠边期)、种子(萌发)及再生苗的茎段诱导愈伤组织,在NT1、NT2和B5三种不同的培养基中进行悬浮培养,筛选出优良的白桦悬浮体系,而后进行单细胞分离的结果表明,白桦花药愈伤组织在B5培养基上生长迅速,分散性好,适合于细胞分离。分离得到的单细胞用不同方式培养,建立了白桦单细胞两步培养法。
In this study, calli of Betula platyphylla were induced from three kinds of explants： anther （microspores in monokaryotic stage）, the seedling, and the stem of in vitro cultured plant. The best growth of calli induced from anther was obtained in the B5 medium when they were suspended in three media： NT1, NT2 and B5, respectively, also in that medium the anther calli shown good dispersivity in liquid medium, which was suited to single cell separation. The single cell was cultured in different ways, and the white birch two-step single cell culture was established.
出处
《植物生理学通讯》
CSCD
北大核心
2010年第4期370-374,共5页
Plant Physiology Communications
基金
东北林业大学本科生创新项目(091022532)
国家自然科学基金(30872045)
关键词
白桦
悬浮培养
优良悬浮体系
单细胞培养
Betula platyphylla
suspension culture
fine suspension system
single cell culture
