高迁移率族蛋白B1和基质金属蛋白酶-9在宫颈鳞癌中的表达及其临床意义

Expressions of High Mobibity Group Box 1 Protein and Matrix Metalloproteinase-9 in Cervical Squamous Epithelial Carcinoma and Their Clinical Significance

目的:研究宫颈鳞癌中高迁移率族蛋白B1(HMGB1)和基质金属蛋白酶-9(MMP-9)表达情况,及其与宫颈癌临床病理特征的关系。方法:应用免疫组化法检测宫颈鳞癌及正常宫颈组织中HMGB1和MMP-9的表达情况。结果:HMGB1在对照组、原位癌和宫颈浸润癌中的阳性率分别为15.8%(3/19)、53.3%(8/15)和80.0%(32/40),P<0.01;HMGB1的表达与宫颈癌临床分期有关(P<0.05),与患者的年龄、病理分级、淋巴结转移无关(P>0.05)。MMP-9在对照组、原位癌和浸润癌中的阳性率分别为21.5%(4/19)、53.3%(8/15)和65.0%(26/40),P<0.01。宫颈癌中HMGB1与MMP-9的共同阳性表达率为47.3%(26/55),rs=0.305,P<0.05。结论:HMGB1与宫颈癌的发生有关,HMGB1与MMP-9表达呈正相关,提示HMGB1可能与宫颈癌的浸润、转移有关。

Objective： To investigate the expressions of High Mobility Group Box 1（HMGB1） and matrix metalloproteinase-9（MMP-9） in human cervical squamous epithelial carcinoma（CSEC）,and to explore the relationship among HMGB1,MMP-9 and clinical pathological characteristics of CSEC.Methods： Immunohistochemisty methods were adopted to detect the expressions of HMGB1 and MMP-9 in cervical squamous epithelial infiltrating carcinoma（CSEIC）,carcinoma in situ（CIS） and normal control groups.Results： The positive expression rate of HMGB1 in CSEIC,CIS and control group was 80.0%（32/40）,53.3%（8/15） and 15.8%（3/19）,respectively,with a significant difference among them（P〈0.01）.The expression of HMGB1 had no relation with age,differentiation degree,lymphatic metastasis or clinical classification（all P〉0.05）.The positive expression rate of MMP-9 in CSEIC,CIS and control group was 65.0%（26/40）,53.3%（8/15） and 21.5%（4/19）,respectively（P〈0.01）.There is a positive correlation between the expression of HMGB1 and MMP-9 in CSEC,and their co-positive rate was 47.3%（26/55,rs=0.305,P〈0.05）.Conclusion： HMGB1 plays a role in the development of CSEC.The expressions of HMGB1 and MMP-9 in CSEC have a positive correlation with each other,which suggests that HMGB1 might be related with the invasive and metastasis of cervical carcinoma.