CD3＋CD56＋自然杀伤T细胞在系统性红斑狼疮中的检测及意义

Clinical significance of CD3＋CD56＋ natural killer T cells in patients with systemic lupus erythematosus

目的 检测系统性红斑狼疮（SLE）患者外周血中自然杀伤T细胞（NKT）的表达,探讨其与SLE疾病活动的关系.方法 入选确诊的SLE患者30例及健康对照30名,采用流式细胞仪检测外周血CD3＋D56＋NKT细胞的表达,分析NKT细胞的表达与SLE疾病活动评分和免疫学指标的相关性,采用两样本均数的t检验和Pearson相关分析进行统计学处理.结果与健康对照（4.16±0.22）%相比,SLE患者外周血中CD3＋CD56＋比例（2.53±0.33）%显著降低,NKT值与血清IgG水平、抗双链DNA抗体定量及SLE疾病活动指数（SLEDAI）评分呈负相关（r值=-0.595,-0.408,-0.637;P均〈0.05）.结论 SLE患者外周血中NKT细胞表达下降,其下降与SLE的疾病活动存在相关性.

Objective To investigate the alteration of NKT cells number in patients with systemic lupus erythematosus （SLE） and its correlation with SLE disease activity.Methods Blood samples were obtained from 30 patients with SLE and 30 healthy control subiects.The number of NKT cells from peripherial blood mononuclear cells （PBMC） were detect by flow cytometry （FCM）.Two independent samples t-test and Pearson correlation was used to evaluated the relationship between SLE disease activity and NKT cells.Results Compared with healthy controls [（4.16±0.22）%],lower number of NKT cells were found in SLE group[（2.53±0.33）%]（P〈0.05）.An inverse correlation was observed between the frequency of NKT cells and IgG,anti-dsDNA,SLE disease activity index.Conclusion Reduced proportions of NKT cells observed in patients with SLE and is associated with high SLE disease activity.