摘要
目的观察大鼠主动脉内皮球囊损伤后血管重塑发生以及血管紧张素转换酶Ⅱ、血管紧张素转换酶表达的变化,探讨缬沙坦对其干预作用及作用机制。方法建立球囊损伤大鼠主动脉内皮损伤模型,并将大鼠随机分为对照组、手术组和缬沙坦治疗组。每组分别于术后14天和30天取主动脉组织,通过逆转录酶连反应技术检测血管紧张素转换酶Ⅱ、血管紧张素转换酶的mRNA水平,应用免疫组化方法检测血管紧张素转换酶Ⅱ、血管紧张素转换酶的蛋白表达。结果主动脉球囊损伤大鼠术后14天和30天血管内膜较对照组均显著增厚,给予缬沙坦干预14天和30天大鼠主动脉血管内膜较手术组明显减轻。手术组较对照组血管紧张素转换酶的mRNA及蛋白表达均显著升高(P<0.05),血管紧张素转换酶Ⅱ的蛋白表达显著降低(P<0.05)。缬沙坦能够降低血管紧张素转换酶的mRNA与蛋白表达(P<0.05),增加血管紧张素转换酶2的蛋白表达(P<0.05)。结论缬沙坦逆转主动脉内皮球囊损伤后血管重塑可能与增加血管紧张素转换酶Ⅱ表达和降低血管紧张素转换酶表达有关。
Aim To investigate the expression of angiotensin-coverting enzyme（ACE）,angiotensin-coverting enzyme Ⅱ（ACEⅡ） and the effects of Valsartan on them after vascular balloon injury in rats.Methods Rat models of aortic endothelial denudation were made by an 2F balloon catheter.Rats were randomly allocated into three groups： Group 1 served as controls;Group 2 were given vascular balloon injury;Group 3 were given Valsartan from 1day before injury to 30 days after injury.The expression of ACE mRNA and the level of protein of ACE or ACEⅡ were investigated at day 14 and 30 after injury by reverse transcription polymerse chain reaction（RT-PCR） technique and immunohistochemistry method,respectively.Results Intimal thickening was significant at 14 days and 30 days after injury.Valsartan significantly and comparably prevented intimal thickening at 14 days and 30 days after balloon injury.The expression of ACE mRNA and protein was significantly increased at 14 days and 30 days after injury（P0.05）,while the expression of ACEⅡ protein was significantly increased at 14 days and 30days after injury（P0.05）.Valsartan can decrease the expression of ACE mRNA and protein and increase the expression of ACEⅡ protein（P0.05）.Conclusion The effects of Valsartan on aortic endothelial denudation and regression of vascular remodeling are related with the increased expression of ACEⅡ protein and the decreased expression of ACE.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第2期109-113,共5页
Chinese Journal of Arteriosclerosis
基金
青岛市科学技术局资助项目(编号02-2-kj-yn-25)
