维吾尔族、汉族载脂蛋白B基因EcoRⅠ多态性与冠心病合并2型糖尿病的关系

Relationship Between Coronary Heart Disease with Diabetes and EcoRⅠ Polymorphism of Apolipoprotein B Gene in Uygur and Han Nationality

目的探讨维吾尔族、汉族冠心病及冠心病合并糖尿病患者载脂蛋白B基因EcoRⅠ多态性的的差异。方法选取冠心病患者266例,其中合并糖尿病154例;对照组136例。用聚合酶链反应和连接酶法检测载脂蛋白B基因EcoRⅠ多态性基因型及等位基因频率分布,研究载脂蛋白B基因EcoRⅠ多态性对血脂、载脂蛋白水平的影响。结果冠心病组及冠心病合并糖尿病组载脂蛋白B基因EocR1基因型及等位基因频率与对照组差异无显著性,在维吾尔族和汉族人群中差异亦无显著性。冠心病合并糖尿病组不同基因型间血脂、载脂蛋白水平差异无显著性。结论载脂蛋白B基因EcoRⅠ多态性对血脂水平有一定影响,但不是汉族、维吾尔族冠心病及冠心病合并糖尿病发病的独立危险因素。

Aim To investigate the association between apolipoprotein B（ApoB） gene polymorphism and coronary heart disease with diabetes in Han and Uygur.Methods Using PCR-LDR,we detected the genotypes of EcoRⅠ of ApoB gene in 136 unrelated healthy individuals and 266 patients with evidence of CHD,which included 154 diabetes.The relation of gene polymorphism ApoB and levels of serum lipids,apolipoproteins was also studied.Results Neither the frequencies of genotypes nor those of alleles of ApoB gene EcoRⅠ polymorphism was statistically different between patients and controls（P0.05）,both in Uygur and Han in CHD and CHD with diabetes.No relation was found between A allele and lipid level.Conclusion The EcoRⅠ polymorphism of ApoB gene maybe affect the levels of serum lipids and lipoproteins,but were not major genetic factors of CHD and CHD with dibetes in Han and Uygur.