RP-HPLC法测定阿托伐他汀在新西兰兔体内的药代动力学

Pharmacokinetics of Atorvastatin in Plasma of New Zealand Rabbits by High Efficiency Liquid Chromatography

目的:研究阿托伐他汀片在新西兰兔体内的药代动力学。方法:18只成年健康雄性新西兰兔,随机分为正常对照组、10mg/kg·d阿托伐他汀片组与15mg/kg·d阿托伐他汀片组,每组6只,采用RP-HPLC法测定血药浓度,计算药代动力学参数。结果:10mg/kg·d组与15mg/kg·d组的主要药代动力学参数分别为:AUC0～t/μg·L-1·h为(619.58±215.45)与(1138.34±422.32)、AUC0～∞/μg·L-1·h为(655.68±242.83)与(1216.57±353.64)、Cmax/μg·L-1为(455.81±168.52)和(896.53±168.5.8)、MRT0～t/h为(3.68±0.75)与(5.73±0.56)、MRT0～∞/h为(3.83±0.91)与(5.25±0.48)、Tmax/h为(2.51±0.82)与(3.68±0.33)、T1/2/h为(4.22±0.55)与(9.51±0.67)。结论:RP-HPLC法适用于阿托伐他汀片动物药代动力学的研究。

Objective：To study pharmacokinetics of atorvastatin tablets in plasma of New Zealand rabbit by High efficiency liquid chromatography（HPLC） method.Methods：Eighteen rabbits were divided three groups：Control group,10 mg/kg·d group and 15 mg/kg·d group.A single dose atorvastatin tablets was given to 12 rabbits.The pharmacokinetics were measured.Results：The main pharmacokinetic of 10 mg/kg·d group and 15 mg/kg·d group test tablets were as follow：AUC0～t/μg·L-1·h were（619.58±215.45）and（1138.34±422.32）,AUC0～∞ /μg·L-1·h were（655.68± 242.83） and（1216.57±353.64）,Cmax/μg·L-1 were（455.81±168.52） and（896.53±168.5.8）,MRT0～t /h were（3.68±0.75） and（5.73±0.56）,MRT0～∞ /h were（3.83±0.91） and（5.25±0.48）,Tmax /h were（2.51±0.82） and（3.68±0.33）,T1/2 /h were（4.22±0.55） and（9.51±0.67）.Conclusion：The method is simple and reproducible,and is suitable for content determination and pharmacokinetic of atorvastatin.