3种制备方法对血小板活化状态的影响

Effects of three different platelet preparation methods on platelet activation

目的:研究富血小板血浆法(PRP)、白膜回浆法(BC)和血细胞分离机(Trima)单采3种制备方法对血小板活化状态的影响。方法:采用流式细胞术和酶联免疫吸附法分别检测3种方法制备的血小板表面CD62P和血浆可溶性CD62P(sCD62P)的表达。结果:Trima制备的单采血小板CD62P和血浆sCD62P的表达水平均明显低于BC和PRP制备的浓缩血小板(P<0.01)。在3种血小板制品中,PRP制备的浓缩血小板CD62P和sCD62P的表达水平最高。结论:血细胞分离机(Trima)制备的单采血小板活化水平明显低于BC和PRP制备的浓缩血小板。

Objective：To study activation of platelets caused by different preparation procedures of platelet-rich plasma （PRP）,buffy coat （BC） methods and Trima cell separator.Method：The effects of different methods on activation of platelets were measured by flow cytometric analysis of CD62P expression.The plasma-soluble CD62P （sCD62P） levels were quantified by enzyme-linked immunosorbent assay.Result：Lower levels of CD62P expression and sCD62P secretion were observed in Trima products than those in BC-PC （platelet concentrates） and PRP-PC preparations （P0.01）.PRP-PC had the highest expression levels of CD62P and sCD62P in the three types of PC.Conclusion：Platelets obtained with the Trima cell separator were significantly less activated than platelets harvested with the BC and PRP methods.