“福星01”人参与黄果人参及人参农家类型的ISSR、RAMP分析

Genetic Diversity of Fuxing 01,Huangguo Ginseng and Five Land Races of Panax ginseng Based on RAMP and ISSR Markers

利用RAMP和ISSR2种标记分别对人参品种及农家类型间的遗传差异进行检测和分析。结果表明:2种标记均能揭示各材料间的遗传多样性,其中RAMP标记的多态性高于ISSR标记。在ISSR标记中,每个引物可扩增出1～7条DNA片段,24个引物扩增出97条带,平均为4.04条。其中45条带具有多态性,多态性比率为46.4%,揭示供试材料间遗传相似系数(GS)为0.7526～0.9691,平均值为0.8304。在RAMP分析中,每条引物可扩增出1～8条DNA片段,23对引物共扩增出112条带,平均为4.87条。其中75条带具有多态性,多态性比率66.9%,揭示供试材料间遗传相似系数(GS)为0.5625～0.9286,平均值为0.7114。聚类分析结果表明,2种标记均能将供试材料完全分开,分类具有一定的相似性,即聚类与地域性有一定关系。

The genetic diversity and genetic relationships among Panax ginseng varieties fuxing 01 and huangguo,five land races of Panax ginseng,namely,damaya,yuanlu,dazhujielu,xiaozhujielu and caolu were evaluated by RAMP and ISSR markers,and RAMP was superior to ISSR.In ISSR analysis,a total of 97 bands were detected,among which 45 bands were polymorphic.The number of bands from each ISSR marker ranged from 1 to 7,with an average of 4.04.The percentage of polymorphic bands was 46.4%,ISSR-derived genetic similarity ranged from 0.752 6 to 0.969 1,with a mean of 0.830 4.In RAMP analysis,a total of 112 bands were observed in 23 markers,among which 75 bands were polymorphic.Each primer could amplify 1 to 8 polymorphic bands,with an average of 4.87 bands.The percentage of polymorphic bands was 66.9%,the RAMP-based genetic similarity ranged from 0.562 5 to 0.928 6,with a mean of 0.711 4.The cluster analysis indicated that all the accessions could be distinguished by both RAMP and ISSR markers.These results suggested that the cluster of accessions was associated with resource region.