HBV基因型在我国九省市的分布及与临床指标的关系

Distribution of HBV genotype in nine provinces of China and relationship between clinical characteristics of patients with HBV infection and genotypes

目的用反向斑点杂交检测HBV基因型的方法 ,了解我国HBV基因型的分布及其临床意义。方法利用反向斑点杂交对我国云南、江苏、浙江、新疆、北京、辽宁、福建、湖南、广东等9个省市的HBV阳性患者血清,和广州地区的肝癌患者术前血清进行HBV基因分型,并结合其临床指标进行相关性分析。结果 706例被分型的标本中,A型占0.1%(1/706)、B型占46.2%(326/706)、C型占49.0%(346/706)、D型占1.6%(11/706)、混合感染占3.1%(22/706)。HBV基因型与患者的年龄、性别、e抗体、e抗原差异没有统计学意义;肝癌患者的HBV基因型与肿瘤包膜、病灶数量、肝炎史、浸润、血管侵入、转移、临床分期、肿瘤家族史、饮酒史以及一些生化指标等差异也没有统计学意义。但发现了HBV基因型与病毒载量之间的关联具有统计学意义(P<0.01)。结论我国HBV基因型以B、C型为主,两者之间的感染人数差别不明显。我国还有少量D型分布,更少量的A型。HBV基因型与HBV病毒载量之间的关联具有统计学意义(P<0.01),提示HBV基因型可能影响患者体内HBV的复制。

Objective To study the distribution of hepatitis B virus（HBV） genotype in China and its clinical significance.Methods HBV positive sera from Yunnan,Jiangsu,Zhejiang,Xinjiang,Beijing,Liaoning,Fujian,Hunan and Guangdong province of China,sera of hepatocellular carcinoma（HCC） patients from Guangzhou city were determined HBV genotype by flow through dot blot assay.The relationship of HBV genotype and clinical characteristics was analyzed.Results 1 genotype A（0.1%）,326 genotype B（46.2%）,346 genotype C（49.0%）,11 genotype D（1.6%） and 22 mixed genotype infection（3.1%） were found.No significance was found between HBV genotype and age,gender,HBeAg,HBeAb.No significance was found between HBV genotype and tumor amicula,number of focus,hepatitis history,infiltrate or not,blood vessel invasion,drink history,tumor family history,metastasis,clinical staging,ALT or AST in HCC patients.But significance difference was found between HBV genotype and HBV DNA level（P0.01）.Conclusion Genotype B and C were both prevalent in China.Genotype D and A were also found in China.Significance difference was found between HBV genotype and HBV DNA level,which suggested replication of HBV in patients might be varied by HBV genotype.