期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

淋巴母细胞端粒酶活性和成瘤性的关系 被引量：2

The relationship of telomerase activity and tumorigenesis of lymphoblastoid cell lines
下载PDF
导出
摘要 目的 EB病毒相关的淋巴母细胞(lymphoblastoid cell lines,LCLs)有较高的端粒酶活性。本实验旨在分析淋巴母细胞成瘤性与端粒酶活性的关系。方法采用端粒重复扩增法(telomericrepeat amplification protocol,TRAP)分别测定淋巴细胞源性肿瘤细胞以及EB病毒相关淋巴母细胞的端粒酶活性,并对淋巴母细胞的成瘤性进行体内体外试验。结果 5株EB病毒相关的LCLs端粒酶活性与细胞传代次数有关,它们都能在免疫缺陷小鼠体内致瘤。结论容易形成集落的细胞系更容易在小鼠体内致瘤,也具有更高的端粒酶活性。 Objective It is believed that lymphoblastoid cell lines（LCLs） associated with Epstein-Barr virus（EBV） are companied with the high telomerase activity.This article is mainly about the relationship of the tumorigenic cell line and telomerase activity.Methods Clone forming test of 5 LCLs were conducted on agar plate and their telomerase activity were analyzed using an in situ telomeric repeat amplification protocol（TRAP）.Results 5 LCLs have the different telomerase activity.All of LCLs can lead tumors in NOD-SCID mice.Conclusion Those cell lines with higher telomerase activity are easier to form clone in mice,and can develope into tumor more easily.
作者 张彦 冯哲玲 熊建军 王嘉励 朱振宇
机构地区 广东省妇幼保健院 中山大学基础医学院生化教研室
出处 《分子诊断与治疗杂志》 2010年第3期156-159,共4页 Journal of Molecular Diagnostics and Therapy
基金 国家自然科学基金(30672358)
关键词 EB病毒 转化细胞系 端粒酶 Epstein-Barr virus Transformed cell lines Telomerase
分类号 R733.1 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1Lin K W,Yan J.The telomere length dynamic and methods of its assessment[J].J Cell Mol Med,2005,9(4):977-989.
  • 2杨静,邓琳,丁琳,柳息洪,曹亚.EB病毒活化端粒酶与p16^(INK4A)/E2F1调控的关系[J].现代肿瘤医学,2003,11(3):164-165. 被引量：2
  • 3Ohyashiki K,Ohyashiki J H,Nishimaki J,et al.Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay[J].Cancer Res,1997,57:2100-2103.
  • 4张彦,冯哲玲,熊建军,王嘉励,杨光,乔雯雯,朱振宇.EB病毒相关淋巴瘤动物模型的建立以及肿瘤治疗的初步探讨[J].现代医学仪器与应用,2008,0(1):8-11. 被引量：4
  • 5Nilsson K.Human B-lymphoid cell lines[J].Hum Cell,1992,5,25-41.
  • 6Mochida A,Gotoh E,Senpuku H,et al.Telomere size and telomerase activity in Epstein-Barr virus(EBV)-positive and EBV-negative Burkitt's lymphoma cell lines[J].Arch Virol,2005,150(10):2139-2150.
  • 7Sugimoto M,Tahara H,Ide T,et al.Steps involved in immortalization and tumorigenesis in human B-lymphoblastoid cell lines transformed by Epstein-Barr virus[J].Cancer Res,2004,64(10):3361-3364.
  • 8Kennedy G,Komano J,Sugden B.Epstein-Barr virus provides a survival factor to Burkitt's lymphomas[J].Proc Natl Acad Sci U S A,2003,100(24):14269-14274.
  • 9Takahashi T,Kawabe T,Okazaki Y,et al.In vitro establishment of tumorigenic human B-lymphoblastoid cell lines transformed by Epstein-Barr virus[J].DNA Cell Biol,2003,22(11):727-735.
  • 10Engelhardt M,Mackenzie K,Drullinsky P,et al.Telomerase activity and telomere length in acute and chronic leukemia pre Or post-ex vivo culture[J].Cancer Res,2000,60:610-617.

二级参考文献16

  • 1陈步云,朱乃硕.严重联合免疫缺陷小鼠在肿瘤与免疫研究中的应用[J].河北医科大学学报,2005,26(4):316-320. 被引量：6
  • 2宋立兵,李隽,曾木圣,张玲,廖雯婷,李满枝,郑美莲,汪慧民.EB病毒的BARF1基因在人支气管上皮细胞恶性转化和肿瘤形成中的作用[J].肿瘤,2006,26(1):10-13. 被引量：1
  • 3[1]Gregory Kennedy,Jun Komano.Bill Sugden Epstein-Barr virus provides a survival factor to Burkitt's lymphomas[J].Proc Natl Acad Sci USA,2003,100(24):14269-14274.
  • 4[2]Woodford N L,Call D R,Remick D g,et al.Model of angiogenesis in mice with severe combined immunodeficiency (SCID)and xenoengrafted with Epstein-Barr virus transformed B cells[J].Comp Med,2004,54(2):209-15.
  • 5[3]Molinie V,Pouchot J,Navrati I E,et al.Primary Epstein-Barr virus related non-Hodgkin's lymphoma of the pleural cavity following long standing tuberculous empyema[J].Arch Pathol lab Med,1996,120:288.
  • 6[8]Ngan Lam,Mark L,Sandberg et al.High Physiological Levels of LMP1 Result in Phosphorylation of eIF2α in Epstein-Barr Vires-Infected Cells[J].J Virol,2004,78(4):1657-1664.
  • 7[10]Popescu I,Macedo C,Zeevi A,et al.Ex vivo priming of naive T cells into EBV-specific Th1/Tc1 effector cells by mature autologous DC loaded with apoptotic/necrotic LCL[J].Am J Transplant,2003,3(11):1369-1377.
  • 8[11]Barbara Savoldo,Helen Huls,Zhensheng Liu,et al.Autologous Epstein-Barr virus(EBV)-specific cytotoxic T cells for the treatment of persistent active EBV infection[J].Blood,2002,100(12):4059-4066.
  • 9[13]Molinie V,Pouchot J,Navratil E,et al.Primary Epstein-Barr virus related non-Hodgkin's lymphoma of the pleural cavity following long standing tuberculous empyema[J].Arch Pathol Lab Med,1996,120:288-291.
  • 10[14]Nilsson K.Human B-lymphoid cell lines[J].Hum Cell,1992,5:5.41.

共引文献4

同被引文献42

  • 1何迎春,田道法,卢芳国,毛积芳.人突变型p53和EB病毒LMP1转基因小鼠的建立[J].第四军医大学学报,2006,27(1):6-9. 被引量：18
  • 2Sun L,Che K,Zhao Z, et al. Sequence analysis of Epstein- Barr virus (EBV) early genes BARF1 and BHRF1 in NK/T cell lymphoma from Northern China[J]. Virol J, 2015,12(1) :135.
  • 3Cohen,JI. Epstein-Barr virus vaccines[J]. Clin Transl Im- munology,2015,4(1) :e32.
  • 4Takashima K, Ohashi M, Kitamura Y, et al. A new animal model for primary and persistent Epstein-Barr virus infec- tion: human EBV-infected rabbit characteristics deter- mined using sequential imaging and pathological analysis [J]. J Med Virol,2008,80(3) :455-466.
  • 5Kanai K, Kato K, Sano H, et al. In vitro Epstein-Barr vi- rus infection model of rabbit lymphocytes from peripheral blood or spleen[J]. Intervirology,2011,54(1) ..17-24.
  • 6Niedobitek G, Agathanggelou A, Finerty S, et al. Latent Epstein-Barr virus infection in cottontop tamarins. A possible model for Epstein-Barr virus infection in humans [J]. Am J Pathol,1994,145(4) :969-978.
  • 7Cleary ML, Epstein MA, Finerty S, et al. Individual tumors of multifocal EB virus-induced malignant lymphomas in tamarins arise from different B-cell clones [J]. Science, 1985, 228 (4700) :722-724.
  • 8Gregorovic G, Bosshard R, Karstegl CE, et al. Cellular gene expression that correlates with EBER expression in Epstein-Barr Virus-Infected lymphoblastoid cell lines[J]. J Virol,2011,85(7) :3535-3545.
  • 9Mosier DE, Gulizia RJ, Baird SM, et al. Transfer of a functional human immune system to mice with severe combined immunodeficiency [ J ]. Nature, 1988, 335 (6187) : 256-259.
  • 10Melkus MW, Estes JD, Padgett-Thomas AA, et al. Hu- manized mice mount specific adaptive and innate immune responses to EBV and TSST-1 [J]. Nat Med, 2006, 12 (11) .- 1316-1322.

引证文献2

分子诊断与治疗杂志
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部