基于酶速率法测定血浆同型半胱氨酸研究

A study on determination of plasma homocysteine by enzyme Kinetic method

目的：通过建立简便的酶速率法，对血浆同型半胱氨酸进行直接检测。方法：使用AU2700全自动生化分析仪，采用两点酶速率法，主波长341nm，副波长406nm，温度37℃，两点线性校准。结果：本法线性范围1．0～51．0μmol／L；日内CV为2．0％～3．1％，日间CV为4．1％～5．8％；低、中、高浓度的回收率分别为102．6％，99．4％，95．9％，平均为99．3％；酶速率法与Abbott Axsym免疫分析仪检测法比较，Y=1．018X-0．865，r^2=0．965。结论：酶速率法简便、快速、敏感、可靠，适用于临床实验室常规和自动化分析，值得推广应用。

Objective： To establish a simple enzyme kinetic method for measuring homocysteine in plasma directly. Methods： Two-point enzyme kinetic method was applied to analyze plasma homocysteine by using AU2700 au -tomatic biochemistry analyzer, The assay parameters are as followings： The dominant wavelength was at 340nm, The subsidiary wavelength was at 405nm, Its temperature at 37 ℃, and two-point linear adjustment. Results： The linearity ra -nge was 1.0 - 51.01amol/L; The CVs of within-day were 2.0% - 3.1%, The CVs of between-day were 4.1% - 5.8%; The recovery rate on low, medial and high consistence were 102.6%, 99.4% and 95.9% respectively, The average recovery rate was 99.3%; The enzyme kinetic method was compared with the Abbott Axsym immunoluminous analyzer, Y= 1.018X-0.865, r^2=0.965. Conclusions： The enzyme kinetic method determination of plasma homocysteine was sim -pie, rapid, sensitive and reliable, It was suitable for the routine automatic analysis and to be applied in clinical practice. Research