优势菌群失衡对肠黏膜上皮结构的损害

The influence of predominant flora dysbacteria on intestinal constructure

目的通过观察小鼠肠道优势菌群失衡肠黏膜上皮结构的变化以探讨肠道优势菌群失衡对黏膜机械屏障的影响。方法利用光镜及电镜技术观察轻度、重度菌群失衡小鼠肠道黏膜绒毛形态变化及上皮细胞超微结构的变化。结果菌群失衡小鼠肠黏膜绒毛出现肿胀、断裂,绒毛顶端肠上皮细胞坏死、脱落,重度菌群失衡与轻度菌群失衡小鼠比较绒毛结构受损加重。超微结构观察发现上皮细胞间隙增宽,胞浆内出现空泡结构,黏膜及黏膜下层有淋巴细胞浸润。结论抗生素干扰肠道优势菌群,可导致肠道机械屏障黏膜绒毛及超微结构受损且重度优势菌群失衡的损害大于轻度优势菌群失衡的损害。

Objective To study morphological constructure changes of gastrointestinal mucosa in mice with intestinal dysbacteria and its effect on intestinal physical barrier.Method Morphological constructure changes of gastrointestinal mucosa were observed by optical microscope and transmission electron microscope.Result The intestinal mucosal villi were damaged with swelling and breakages in it;the gaps of intestinal epithelial cells were wider;cells showed necrosis and empty vacuoles;lymph cells infiltrated mucosa layer.The damage was more serious in mice with heavy-dysbacteria than in light-dysbacteria.Conclusion Intestinal dysbacterial can result in damage to mucosal villi and decrease in the level of intestinal mucosal immunity in mice.