新疆伊犁地区驴脑组织亨德拉病毒感染情况调查

Detection of Hendra virus infection in donkey brain tissues in Ili,Xinjiang

目的调查新疆伊犁地区草原放养驴群中亨德拉病毒(Hendra virus,HeV)中枢感染的流行情况。方法采用一步法实时荧光定量RT-PCR检测病毒核酸片段的方法对采自新疆伊犁地区草原放养、未接种HeV疫苗的65例驴脑组织进行HeV核蛋白(N)基因片段检测。结果65例驴脑组织成功进行一步法实时荧光定量RT-PCR检测,未检出阳性样本。结论目前尚未发现我国新疆伊犁地区放养驴中存在HeV中枢神经感染的证据,提示该地区出现HeV感染流行的可能性不大。

Objective To detect Hendra infection in the brain tissue of grazing donkeys,in Ili,Xinjiang,and investigate the prevalence of HeV in this area.Method HeV nucleoprotein（N） gene fragment was detected by one-step real-time RT-PCR in 65 donkeys brain samples from Ili grassland,Xinjiang.Result The method was capable specifically of detecting HeV RNA in donkey brain tissues.No positive RNA sample was detected in these samples.Conclusion There is no evidence of HeV infection in grazing donkeys in Ili,Xinjiang in this preliminary epidemiology study,which suggests that the outbreak of Hendra virus infection in this region is impossible in the near future.