漏声表面波生物传感器直接快速检测病原体DNA的实验研究

Rapid Detection of Pathogen DNA Using Leaky Surface Acoustic Wave Peptide Nucleic Acid Biosenser

目的应用漏声表面波生物传感器直接检测从临床标本中提取的HPV基因组DNA。方法选取36例临床确诊为宫颈癌的患者,提取其生殖器分泌物的基因组DNA,并经荧光定量PCR检测为HPV阳性的标本,以14例健康志愿者为阴性对照;利用漏声表面波生物传感器检测临床样本,并与荧光定量PCR法进行方法学比较。结果传感器法检测35例标本为阳性,阳性率为97.22%,而荧光定量PCR法检测36例均为阳性;14例阴性对照标本中,两种方法的检测结果均为阴性;传感器法的检测限为1.21pg/L。结论传感器法与荧光定量PCR法差异无统计学意义,一致性较好,且漏声表面波生物传感器实现了对临床标本中HPV基因组DNA的直接快速检测。

OBJECTIVE To directly detect HPV genomic DNA extracted from clinical samples with leaky surfaqe acoustic wave （LSAW） biosensor. METHODS Extracted genomic DNA from 36 clinical positive samples diagnosed for HPV infection and 14 negative controls samples were detected with LSAW biosensor and quantitative PCR, respectively. The advantages and disadvantages of both methods were summarized. RESULTS Among 36 clinical cases being diagnosed for HPV infection by PCR, 35 samples were detected correctly by the LSAW biosensor. The positive rate was 97.22%. The HPV was not detected in the 14 controls with either of two methods. The detection limit was 1.21 pg/L. CONCLUSIONS There is no significant difference between LSAW biosensor and fluorescence quantitative PCR method according to the statistic analysis. HPV genomic DNA from clinical samples can be directly detected with LSAW bis-PNA biosensor.