摘要
目的 观察阿托伐他汀对P-selectin表达及正常血脂的影响.方法 将SD大鼠随机分为假手术对照组（sham）、脑缺血再灌注组（CIR）、阿托伐他汀组（ATO）.阿托伐他汀采用灌胃的方式给药.给药时间分为4、2周、3d、造模后即时组四个亚组,每亚组分别于造模后12、24h断头.断头取脑,制作标本,连续切片作P-selectin免疫组化染色,并在干预给药前、后分别抽血,酶法测定血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）及低密度脂蛋白胆固醇（LDL-C）含量.结果 缺血再灌注6h亚组可见P-selectin阳性表达血管数开始上升,12h达到高峰,至第3天仍有少量表达.在造模前4、2周、3d、造模后立即等不同时间内将阿托伐他汀进行干预后,P-selectin表达明显下调（P〈0.01）,组内两两比较亦有统计学差异（P〈0.01）,阿托伐他汀给药前后各血脂成分均无统计学差异（P〉0.05）.结论 脑缺血再灌注后P-selectin在缺血周围血管内皮表达,使用阿托伐他汀干预后P-selectin含量明显下降,且对正常血脂没有影响.
Objective To investigate of atorvastatin on P-selectin expression after cerebral ischemia-reperfusion in normolipidemic rats. Methods Male SD rats were divided into three groups： cerebral ischemia-reperfusion group （I/R）, sham operation group （S）, atorvastatin group （A） in random. Atorvastatin was given by gastric irrigation for 3d, 2 weeks and 4 weeks before focal cerebral ischemia was induced. Rats were sacrificed after 12 and 24 h of cerebral I/R. Blood samples were taken before and after treatment with atorvastatin for the determination of serum concentrations of total cholesterol, triglyceride, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and low density lipoprotein. Results The expression of P-selectin was increased in endothelium of peripheral vessels at 6h after ischemia reperfusion, peaked at 12h and the low level of expression was still detected at 3d. P-selectin expression was decreased after treatment of atorvastatin in all subgroups（ P〈 0.01 ）,however there were no significant differences in blood lipid levels before and after atorvastatin treatment （P 〉0.05）. Conclusion Atorvastatin can downregulate the expression of P-selectin after cerebral ischemia-reperfusion, but has no effect on the blood lipid levels of normolipidemic rats.
出处
《浙江医学》
CAS
2010年第5期688-689,775,I0003,共4页
Zhejiang Medical Journal
