响应面法优化红曲色素的提取工艺及其稳定性研究

Optimization of extraction process by response surface methodology and stability of monascus pigment

以红曲粉为原料,在单因素实验的基础上,利用Box-Benhnken中心组合设计和响应面分析法对红曲色素的提取工艺参数进行优化研究,实验表明:在乙醇浓度为58.5%、提取时间为75.7min、提取温度为70℃、提取pH为4.3时,红曲色素提取率最高,为87.34%。同时考虑光照、温度、加热时间、pH、金属离子、防腐剂、酸度调节剂、护色剂、抗氧化剂等因子的影响作用,对红曲色素稳定性进行了研究,结果表明:光照和Fe^(3+)对红曲色素稳定性的影响较大,而山梨酸钾、苯甲酸、柠檬酸和KNO_3对红曲色素有护色作用。

With red kojic rice powder as raw material, on the basis of single-factor experiments, the extraction technique parameters of monascus pigment were optimized with Box-Benhnken design and response surface methodology（RSM）. It was indicated that the rate of extraction was 87.34% when the optimum extraction parameters were concentration of alcohol 58.5 %, extraction time 75.7min, temperature 70℃, pH4.3. Futhermore, the stability of monascus pigment were studied in many factors such as illumination, temperature, heating time, pH, metallic ion, antiseptic, acidity regulator, color retention agent, antioxidant. The results showed the effect of illumination and Fe3＋ on the stability of monascus pigment was larger comparatively,whereas potassium sorbate, phenylformic acid,citric acid and KNO, can preserve the color of monascus piqment.