基于红外光谱的花椒蛋白质含量的快速检测

Rapid detection of protein content in Zanthoxylum bungeanum Maxim by infrared spectroscopy

为了开发花椒品质的快速检测新技术,本文用红外光谱漫反射技术对不同品种花椒的蛋白质含量进行了检测。首先采用常规的凯氏定氮仪测试了花椒样品的蛋白质含量,同时采集该样品的红外漫反射光谱,并进行二阶导数预处理,再分别用多元线性回归、主成分分析和偏最小二乘法进行回归分析。结果表明,多元线性回归法优于其他两种方法,其决定系数、剩余标准差分别为0.9317、0.1658。因此,多元线性回归法是花椒蛋白质含量红外检测的最优建模方法。

The protein content in different kinds of Zanthoxylum bungeanum Maxim were measured by infrared reflectance spectroscopy to develop a new rapid detection technique about the quality of Zanthoxylum bungeanum Maxim.First, used traditional method--gerhard kjedahl determination device to determine the protein content in Zanthoxylum bungeanum Maxim,collected the spectrum of the sample and applied second derivative to pre-treat the spectrum. Then, the regression analysis was made basing on multiple linear regression （MLR）, principal component analysis （PCA） and partial least squares （PLS） respectively. The results indicated that the model developed by MLR was better than others for protein,the coefficient of determination was 0.9317,and the residual standard deviation was 0.1658.Hence, MLR was the best model method of detecting the protein content in Zanthoxylum bungeanum Maxim by infrared spectroscopy.