出芽短梗霉发酵液中聚苹果酸测定方法的比较

Comparison of determination methods for poly(β-L-malic acid) in culture broth produced by Aureobasidium pullulans

应用α-2,7-萘二酚法和酶试剂盒法分别对L-苹果酸标准品和出芽短梗霉(Aureobasidium pullulans)发酵液中的聚苹果酸进行检测,比较二者的差异,并研究了酸解过程对两种检测方法的影响。结果表明:检测L-苹果酸标准品,α-2,7-萘二酚法回收率为99.58%～100.1%,相对标准偏差为0.71%～0.95%,酶试剂盒法回收率为99.6%～100.22%,相对标准偏差为0.55%～0.6%,经统计学分析两种方法的准确度和精密度无显著性差异;检测发酵液中聚苹果酸,α-2,7-萘二酚法相对标准偏差为2.49%～3.61%,酶试剂盒法相对标准偏差为0.81%～1.18%,经统计学分析两种方法的准确度和精密度无显著性差异;酸解过程对两种检测方法均无显著影响。

α-2,7-hydronaphthoquinone method and L-MALATE kit method were used for L-malic acid and poly （β-L-malic acid） in culture broth produced by Aureobasidium pullulans,as well as the influence of acidolysis on the two determination methods.The results showed that the return-ratio and relative standard deviation of α-2,7- hydronaphthoquinone method were 99.58%- 100.1% and 0.71 %-0.95%, respectively, the return- ratio and relative standard deviation of L-MALATE kit method were 99.6%-100.22% and 0.55%-0.6%, respectively, there were no significant different in the accuracy and precision of the two methods.Results of determination of poly （13-L-malic acid） in culture broth by these methods were as follows： the relative standard deviation of α- 2, β- hyd ronaphthoquinone method and L- MALATE kit method was 2.49 %--3.61% and 0.81%~ 1.18 %, respectively, there were no significant different in the accuracy and precision of the two methods. Acidolysis had neither notable infhfluence on the α-2 β-hydronaphthoquinone method nor L-MALATE kit method.