黄瓜耐低温基因转化后代的生物学鉴定

Biological Identification of Low Temperature Tolerance Gene Transform of Cucumber and Its Progeny

对耐低温基因BnCS转化黄瓜植株T0种子及T1植株进行生物学鉴定,结果表明,经低温胁迫,黄瓜品系yd-36的转基因植株T0种子13℃的发芽势、发芽率及胚根长均显著高于非转基因种子;T1植株的电解质渗漏率和丙二醛含量也显著低于非转基因植株,株高、茎粗和叶面积的生长量均高于非转基因植株。证明转基因植株耐低温能力明显强于非转基因植株。

Low temperature tolerance gene BnCS was transformed into cucumber（Cucumis sativus L.）plants.Biological characteristics of transgenic plant（T0）and its progeny（T1）were biologically identified.Under low temperature stress at 13 ℃,the germination potential,germination percentage and radicle length of transgenic T0 seed were significantly higher than the CK（cultivar yd-36）.The percentage of electrolyte leakage and MDA content（T1）were significantly lower than the CK.While the increment growth of plant height,stem width and leaf area were higher than the CK.This indicated that low temperature tolerance of transgenic plants was obviously higher than that of the non-transgenic plants.