摘要
采用抑制差减杂交(SSH)技术,构建甘蓝抗病品种A21经黑腐病菌诱导6、8、12、24h后的混合SSH文库。经反向Northern筛选,共获得87条高质量ESTs。通过NCBI中BLAST数据库比对分析发现,83个ESTs与已知蛋白或基因具有不同程度的同源性。
In this research,suppression subtractive hybridization（SSH）was adopted to construct a mixed SSH library by inducing black rot to disease resistant cabbage（Brassica oleracea L.）variety A21 for 6h,8h,12h and 24h.Through reverse northern screening,87 ESTs with high quality were obtained.After conducting comparative analysis with BLAST data bank in NCBI,83 of the ESTs were found to have homology by different degrees with known protein or genes.
出处
《中国蔬菜》
北大核心
2010年第10期20-24,共5页
China Vegetables
基金
国家"863"计划(2006AA100108-4-9)
关键词
甘蓝
黑腐病
SSH
Brassica oleracea
Black rot
SSH
