硫酸铟对V79细胞氧化损伤及VitC拮抗作用体外研究

Study of Oxidative Damage of V29 Cell Induced by Indium Sulfate and Antagonistic Effect of Vitamin C in vitro

目的观察硫酸铟对中国仓鼠肺成纤维细胞(V79)的氧化损伤及维生素C的修复作用。方法以体外培养的V79细胞为研究对象,四氮唑蓝比色分析法(MTT法)观察不同染毒剂量(0.5、1、2、4、8 mmol/L硫酸铟)和不同染毒时间(2、6、122、4 h)的细胞毒性;应用生化方法检测经铟染毒的细胞内超氧化物歧化酶(SOD)的活力、丙二醛(MDA)含量及维生素C(VitC)的修复作用。结果当硫酸铟浓度≥1 mmol/L时,各染毒组细胞吸光度值与对照组比较降低,差异有统计学意义(P<0.05)。随着硫酸铟浓度增高,SOD活力降低,MDA含量增加;随着VitC浓度的增加,SOD活力升高,MDA含量减少,但当VitC浓度增至500μmol/L时,V79细胞的SOD活力反而下降,MDA含量增高,与对照组比较,差异有统计学意义(P<0.05)。结论硫酸铟对V79细胞的损伤可能与细胞氧化应激增强有关,低浓度VitC(20、100μmol/L)对该损伤有拮抗作用。

Objective To observe the oxidative damage of V29 cell induced by indium sulfate and antagonistic effect of vitamin C in vitro. Methods V29 cells cultured in vitro were taken as the study subject which were exposed to indium sulfate at different dosages（0.5,1,2,4, and 8 mmol/L）for different exposure periods（2,6,12,and 24 h）. The cyto-toxicities were observed with MTT colorimetric assay. The activities of SOD（superoxide dismutase）, contents of MDA （malondialdehyde）in exposed cells and the repair effect of vitamin C were investigated by biochemical method. Results When the concentrations of indium sulfate ≥ 1 mmol/L, the absorption of cells in various exposed groups lowered compared with the control group. The difference was significant（P〈0.05）. The SOD activities reduced and the levels of MDA increased with the increase of indium sulfate concentrations. The SOD activities increased and the levels of MDA reduced with the increase of the vitamin C concentrations. However, as the concentration of vitamin C increased to 500μmol/L, on the contrary, the SOD activities lowered and the levels of MDA of V29 cells increased. The differences were all significant（P〈0.05）. Conclusions The V79 cell damage induced by indium sulfate might correlate with the increase of the oxidative stress. Low concentration vitamin C（20 and 100 μmol/L）might have antagonistic effect on the damage.