超声波辅助提取枇杷叶中的熊果酸的工艺研究

Study on the ultrasonic-assisted extraction technology of ursolic acid from loquat leaf

为了充分利用枇杷中的熊果酸,以枇杷叶为原料,以熊果酸提取率为指标,采用超声波辅助提取了枇杷叶中的熊果酸。分别研究了乙醇浓度、料液比、超声波时间和超声波温度对熊果酸提取的影响,通过正交实验优化了提取工艺。结果表明,熊果酸提取的最佳工艺条件是:乙醇浓度为85%、料液比为1∶12、超声波时间为25 m in、超声波温度为45℃,在最佳条件下,熊果酸提取率可达1.131%。

In order to make a full use of ursolic acid, the ultrasonic - assisted extraction was used to extract ursolic acid from loquat leaf,with loquat leaves as raw materials and the extraction rate of ursolic acid as the indicator. The effects of alcohol concentration, solid- to- liquid ratio, ultrasonic wave time, and ultrasonic wave temperature on the ursolic acid extraction were investigated respectively. The extraction process was optimized through orthogonal test. The final results point out the optimum conditions as follows：alcohol concentration 85% ,ratio of solid to liquid 1： 12,ultrasonic wave time 25 min, and ultrasonic wave temperature 45℃. Under the optimal circumstances,the extraction rate of ursolic acid can be up to 1. 131%.