促表面活性物质治疗豚鼠分泌性中耳炎咽鼓管黏膜组织学观察

HISTOLOGICAL OBSERVATION OF EUSTACHIAN OF GUINEA PIGS WITH SECRETORY OTITIS MEDIA AFTER BEING TREATED BY SURFACTANT AGONIST

目的研究促表面活性物质对分泌性中耳炎的治疗作用,为分泌性中耳炎的治疗探索新的途径。方法采用灭活的肺炎链球菌鼓室内注射,建立分泌性中耳炎模型,并腹腔注射促表面活性物质盐酸氨溴索,观察治疗前后咽鼓管黏膜光镜和电镜的变化。结果造模完成后,鼓膜均变浑浊、充血、光锥消失。咽鼓管黏膜水肿、增生、炎性细胞浸润明显,暗颗粒细胞分泌功能受损,表面活性物质板层体结构明显减少或消失。应用促表面活性物质后,炎症反应减轻,暗颗粒分泌细胞结构近正常,胞浆内有散在分布的板层体样物质。结论促表面活性物质可以改善分泌细胞的病理变化,对豚鼠分泌性中耳炎有治疗作用。

Objective To study the effect of surfactant agonist on secretory otitis media （SOM） in guinea pigs to find a new way to manage it. Methods Nonviable heat-killed pneumoeocci solution was inoculated into the middle ear cavity in guinea pigs to set up a model of SOM. The surfactant agonist （Ambroxol Hydroehloride ） was injected intraperitoneally. The histological changes under light and electron microscope were observed. Results After the SOM models were completed, the tympanums appeared congestive and turbid, the light cone disappeared. The mucosa of eustachian showed edema, hyperplasia and inflammatory cells infiltrating. No surfactant-like lamellar bodies were found in the secretory cells, which showed impaired characters with little secertory granules. After the surfactant agonist was injected, the inflammatory reponse reduced, the structure of dark granular secretory cells appeared normal,there were surfactant-like lamellar bodies scattered in the cytoplasm. Conclusion The surfactant agonist can ease the pathological changes in the secretory ceils and plays an important role in SOM of guines piges.