摘要
目的:对抗癌散的抗肿瘤活性成分进行研究。方法:用硅胶、葡聚糖凝胶(Sephadex LH-20)柱色谱等方法进行分离纯化,通过理化性质及光谱数据确定化合物的结构;采用流式细胞术(FCM)对部分单体化合物进行抗肿瘤活性的测定。结果:分离得到β-谷甾醇(Ⅰ)、正丁基-β-D-吡喃果糖苷(Ⅱ)、人参皂苷Rg_1(Ⅲ)3个化合物;活性实验结果表明,部分化合物具有一定的抗肿瘤活性。结论:本研究初步阐明了复方抗癌散中的主要化学成分,其中化合物Ⅱ为首次从该植物中分离得到;化合物(Ⅱ)和化合物(Ⅲ)对人肝癌细胞(Bel-7402)有较好的抑制活性。
Objective： To study the antitumor activities of the constituents of kang＇ ai powder. Method ： The constituents were iso- lated by column chromatography and identified by physical and spectral analysis. Meanwhile, the antitumor activities of some compounds were evaluated by FCM assay. Result： Three compounds were isolated and identified as, βsitosterol （ I ） , n-butyl-β-D -fructosidepyr- anose （ Ⅱ ） , ginsenoside Rg1 （ Ⅲ） , respectively. Biological screening results demonstrated that some of the tested compounds were exhibited the antitumor activities in vitro. Conclusion： Compounds Ⅱ were isolated from this plant for the first time. Compound Ⅱ , Ⅲhave a better inhibitory activity on cell line Bel-7402.
出处
《中国药师》
CAS
2010年第6期768-770,共3页
China Pharmacist
基金
国家自然科学基金(编号:30772761 308702490)
