HPLC法测定启阳胶囊中淫羊藿苷的含量

Determination of Icariin in Qiyang Capsules by HPLC

目的:建立高效液相色谱法测定启阳胶囊中淫羊藿苷的含量。方法:色谱柱:Kromasil C_(18)柱(250 mm×4.6 mm,5μm),流动相:甲醇-水-醋酸(45:50:5),流速:1 ml·min^(-1),检测波长:270 nm,柱温:室温。结果:淫羊藿苷浓度在0.047 9～0.958 0μg·ml^(-1)范围内线性关系良好(r=0.999 9),平均加样回收率为99.14%,RSD为0.51%(n=6)。结论:本方法简便快捷,适于该制剂的含量测定。

Objective ： To establish a methods for determination of icariin in qiyang capsules by HPLC. Method ： The Kromasil C18 column （ 250 mm × 4. 6 mm,5μm） was used. The mobile phase was methanol-water-acetic acid （45 ： 50：5 ） with 1.0 ml- min - 1 of flow rate at ambient temperature. The detection wavelength was at 270 nm. Result： The linear range of ieariin was 0. 047 9-0. 958 0 μg. ml- 1 （ r = 0. 999 9） . The average recovery was 99. 14% and RSD was 0. 51% （ n = 6） . Conclusion： The method is simple and quick for determination of this preparations.