摘要
Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.
Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.
基金
Supported by the National Natural Science Foundation of China(No.20771082)
the Natural Science Foundation of Tianjin Municipality, China(No.09JCYBJC05800)
the Educational Commission of Tianjin Municipality, China (No.20080509)
