SBPS对荷瘤小鼠癌基因表达的影响

Effect of Scutellaria Barbata Polysaccharides on N-ras,C-myc Oncogene Expression in Mouse HepA Tumour

目的:为探明半枝莲多糖对HepA瘤组织N-ras、c-myc基因表达的影响。方法:运用原位杂交法、SP免疫组化染色技术及多媒体彩色病理图文分析系统测定HepA瘤细胞中N-ras、C-myc mRNA及蛋白的表达量。结果:半枝莲多糖组、猪苓多糖组中N-ras、C-myc mRNA及蛋白的表达量均显著低于荷瘤组(P<0.01),半枝莲多糖组与猪苓多糖组无显著性差异。结论:半枝莲多糖可通过降低N-ras、C-myc mRNA及蛋白的表达,而抑制HepA瘤细胞的增殖。

Objective： To study the effect of SBPS on N-ras,C-myc expression in HepA tumour. Methods：lmmunohistuchemical staining and ISH were used to determine the effect of SBPS on N-ras,C-myc mRNA and protein expression in mouse HepA tumor.Results：The average levels of N-ras and C-myc expression in SBPS group and PUPS group have much difference with control group. That is to say, SBPS and PUPS can significantly reduce the expression of N-ras and C-myc expression in HepA cells. Conclusions：Regulating the N-ras and C-myc oncogene expression the mechanisms ofantitumor activity.