摘要
目的:为探明半枝莲多糖对HepA瘤组织N-ras、c-myc基因表达的影响。方法:运用原位杂交法、SP免疫组化染色技术及多媒体彩色病理图文分析系统测定HepA瘤细胞中N-ras、C-myc mRNA及蛋白的表达量。结果:半枝莲多糖组、猪苓多糖组中N-ras、C-myc mRNA及蛋白的表达量均显著低于荷瘤组(P<0.01),半枝莲多糖组与猪苓多糖组无显著性差异。结论:半枝莲多糖可通过降低N-ras、C-myc mRNA及蛋白的表达,而抑制HepA瘤细胞的增殖。
Objective: To study the effect of SBPS on N-ras,C-myc expression in HepA tumour. Methods:lmmunohistuchemical staining and ISH were used to determine the effect of SBPS on N-ras,C-myc mRNA and protein expression in mouse HepA tumor.Results:The average levels of N-ras and C-myc expression in SBPS group and PUPS group have much difference with control group. That is to say, SBPS and PUPS can significantly reduce the expression of N-ras and C-myc expression in HepA cells. Conclusions:Regulating the N-ras and C-myc oncogene expression the mechanisms ofantitumor activity.
出处
《中国医药导刊》
2010年第5期833-834,共2页
Chinese Journal of Medicinal Guide
基金
黑龙江省普通高等学校青年学术骨干支持计划项目(1151G060)
中国博士后科学基金项目(20090451028)