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实时荧光定量PCR检测乙型肝炎血清DNA的临床观察

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摘要 目的:探讨乙型肝炎患者血清乙型肝炎病毒DNA定量与乙型肝炎标志物关系。方法:采用荧光探针杂交技术为基础的实时荧光定量PCR检测259例乙型肝炎患者血清中DNA含量,与HBV标志物作对比分析。结果:血清HBeAg阳性组HBV-DNA阳性率为98.6%,HBeAb组阳性率为61.5%;HBeAg组阳性率含量明显高于HBeAb阳性组。结论:实时荧光定量PCR检测具有较高的灵敏度和特异性,定量准确、结果可靠,为临床了解病毒复制情况,制定治疗方案及疗效观察提供了确切依据。
作者 邴圣春
出处 《现代医药卫生》 2010年第12期1814-1815,共2页 Journal of Modern Medicine & Health
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