COX-2在CIN及宫颈癌组织中表达的意义

Expression of cyclooxygenase-2 in CIN and cervical carcinoma

目的:探讨前列腺素酶COX-2在宫颈癌及癌前组织中的表达及意义。方法:采用免疫组化SP法、半定量RT-PCR法及Western-blot法检测COX-2在35例宫颈上皮肉瘤样病变(CIN)、40例宫颈癌和5例正常宫颈组织中的表达情况。结果:①经免疫组化检测,COX-2蛋白在正常宫颈组织中不表达,在CIN宫颈组织阳性表达率分别为31.4%,在宫颈癌组织中阳性表达率分别为85.0%。三者之间差异具有显著性(P<0.05)。②经RT-PCR检测,正常宫颈组织中无COX-2 mRNA表达,CIN宫颈组织中COX-2 mRNA相对含量为0.291±0.214,宫颈癌组织中相对含量为0.357±0.173,三者之间差异具有显著性(P<0.05)。③经Western-blot检测,COX-2蛋白在宫颈癌Ⅰ、Ⅱ、Ⅲ期中相对含量分别为40.27±13.67,56.98±21.13和84.21±22.74,三者之间差异具有统计学意义(P<0.05)。结论:COX-2蛋白不表达于正常宫颈组织中。COX-2可能参与了宫颈癌早期的发生发展。宫颈组织中COX-2的表达可作为宫颈癌早期诊断及估计宫颈癌患者预后的一个指标。

Objective：To examine the expression of cyclooxygenase-2（COX-2） in cervical cancer and cervical intraepithelial neoplasia（CIN） and cervical carcinoma,and their possible correlation with carcinogenesis.Methods：The tissue blocks from 35 CIN patients and 40 patients with cervical carcinoma were included in this study,and 5 normal cervical tissues were served as control group.COX-2 proteins were measured by immunohistochemistry and Western-blot.COX-2 mRNA was examined by the reverse transcripton-polymeras chain reaction（RT-PCR）.Results：① By immunohistochemistry,normal cervical tissues did not express COX-2.The expression of COX-2 in CIN and cervical carcinoma were 31.4% and 85.0%,respectively.There was significant difference between the three tissues（P0.05）.② By RT-PCR,there was no expression of COX-2 mRNA in normal cervical tissues.The expression of COX-2 mRNA CIN and cervical carcinoma were 0.291±0.214 and 0.357±0.173,respectively.There was significant difference between the three tissues（P0.05）.③ By Western-blot,the expression of COX-2 in cancer stage I,II and III were 40.27±13.67,56.98±21.13 and 84.21±22.74,respectively.There was significant difference between the three tissues（P0.05）.Conclusion：There is no expression of COX-2 in normal cervical tissues.COX-2 is probably involved in early carcinogenesis of cervical carcinoma.The detection ofCOX-2 expression may play an important role in early diagnosis and evaluation of prognosis in patients with cervical carcinoma.