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透明质酸对小型猪脂肪干细胞增殖活性的影响 被引量：3

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摘要 目的研究透明质酸(简称HA)对体外培养的小型猪脂肪干细胞(简称ADSCs)增殖活性的影响。方法将复苏第5代ADSCs分为4组,即对照组和含3个不同浓度的HA(分别为0.01mg/ml、0.1mg/ml、1.0mg/ml)组。观察各组ADSCs增殖情况,测定细胞贴壁时间,并采用MTT法绘制细胞生长曲线。结果对照组细胞贴壁时间约7～8h,0.1mg/mlHA组最早,约4h,0.01mg/mlHA、1.0mg/mlHA组分别为10、11h,与对照组相比差异有统计学意义(P值均<0.05)。与对照组相比,0.1mg/mlHA组细胞生长速度最快(P<0.05),1.0mg/mlHA组细胞生长最慢(P<0.01)。结论 0.1mg/mlHA可以提高ADSCs增殖活性。
作者 庞雪芬 张志光 燕子
机构地区 山西医科大学生理学系细胞生理学省部共建重点实验室 中山大学光华口腔医学院附属口腔医院
出处 《中国现代药物应用》 2010年第11期65-66,共2页 Chinese Journal of Modern Drug Application
关键词 透明质酸 脂肪干细胞 增殖 组织工程
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献8

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共引文献10

同被引文献64

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  • 2孔清泉,项舟,鲜思平,汪金平,杨志明.自体骨髓间充质干细胞与外源性透明质酸钠修复兔膝关节软骨缺损的研究[J].中国修复重建外科杂志,2004,18(4):318-322. 被引量：13
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引证文献3

二级引证文献13

中国现代药物应用
2010年 第11期

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