棉铃虫核多角体病毒成纤维细胞生长因子受体的获得及初步分析

Isolation and Preliminary Analysis of the Receptor of Helicoverpa armigera NPV Fibroblast Growth Factor

成纤维细胞生长因子(fibroblast growthfactor,FGF)是一类非常重要的调节因子,它主要通过激活其受体再激活信号转导途径来调节细胞的生长、发育和迁移。昆虫杆状病毒如棉铃虫单核衣壳多角体病毒(Helicoverpa armigera nucleopolyhedrovirus,HearNPV)编码FGF并具有趋化宿主细胞的功能。为了深入研究其作用的分子机制,本文根据家蚕的FGF受体Bmbtl和草地贪夜蛾的FGF受体Sfbtl基因序列设计引物,通过RT-PCR从HearNPV的敏感细胞中获得了FGF受体Habtl的部分序列并对其进行了初步研究。序列分析结果显示获得的Habtl序列与Bmbtl和Sfbtl有很高的同源性;通过siRNA抑制试验,发现Habtl在HearNPV FGF趋化和病毒感染过程中发挥着重要作用。

Fibroblast growth factor （FGF） is a key regulator of developmental processes affecting the growth, differentiation, and migration of many cell types by activating the receptor and the signal pathway. Baculoviruses such as Helicoverpa armigera nucleopolyhedrovirus （HearNPV） also encode a FGF homolog show- ing chemotactic activity. Here we cloned and analyzed the partial sequence of a fgf receptor （Habtl） from Helicoverpa zea cell line, which is permission for HearNPV infection, by RT-PCR with the primers designed based on the sequences of the FGF receptor of Bombyx mori and Spodoptera frugiperda （Bmbtl and Sfbtl, respectively）. Se- quence analysis showed that Habtl had a high identity to Bmbtl and Sfbtl. Knock-down of Habtl expression with special siRNA showed that Habtl played an essential role for HearNPV FGF mediated chemotation and in virus infection.