桦褐孔菌发酵液提取物对不同细胞株的药理作用

Effects of Inonotus obliquus's Fermentation Liquid Extraction on Different Cell Lines

本文采用桦褐孔菌发酵液提取物(IOFE)对人肝癌细胞株HepG_2、人胃癌细胞株SGC7901、正常组织来源的人肝细胞HL-7702,进行体外细胞试验,结果表明,IOFE在低浓度处理条件下对HepG_2和SGC7901均有抑制作用,且对SGC7901抑制效果最好;在高浓度条件下对HepG_2和SGC7901的生长具有一定的促进作用;IOFE对氟尿嘧啶损伤后的HL-7702具有非常高的修复作用,随浓度的升高,修复作用逐渐增强,在高浓度3 000μg/ml处理条件下,修复率为303.01%。因此,IOFE对肿瘤细胞的作用表现可知提取物含有复杂的成分,这些成分具有抑制或促进细胞增殖的作用,随着浓度的升高促进作用的成分占优势,但对正常细胞,甚至化疗药物损伤后的细胞却有更好的促进作用。

Fermentation liquid extraction samples of Inonotus obliquus＇s strains （IOFE） were initially chosen to test the cell activity in vitro using hominal＇s liver cancer cell line HepG2, the hominal＇s stomach cancer cell line SGC7901 and the normal hominal＇s liver cell HL-7702. The result indicated that the HepG2 and the SGC7901 could been inhibited in the low consistency, comparatively, the inhibition effect on SGC7901 was better than on HepG2; the extraction not only had no inhibition effect on the multiplication of the HepG2 and the SGC7901, but also had definite auxo-action; Although fermentation liquid extraction of Inonotus obliquus＇s strains had the lower inhibition effect on HepG2 and the SGC7901, it had the greatly repair effect on the postinjury HL-7702, and the repair function increased along with the strengthen of the concentration. In the high concentration 3 000 ~tg/ml, the repair rate was 303.01%. It followed that IOFE could be used as an antineoplastic ancillary drug to repair the greatly injury on patient by the chemotherapeutics in the treatment of oncotherapy.