束缚应激致亚健康状态大鼠氧自由基变化及中药干预作用

Oxygen Radical Changes of Sub-healthy Rat Induced by Restraint Stress and Altered by Traditional Chinese Medicine

目的限制大鼠活动致束缚应激形成大鼠亚健康状态模型，观察亚健康状态大鼠氧自由基变化及中药干预效果。方法雄性SD大鼠40只，体重320—360g，随机分成4组，即正常对照组、模型对照纽、抗衰老片组和六味地黄丸组，每组10只。除正常组外，其它各组每天限制大鼠活动3h，形成束缚应激，建立亚健康状态大鼠模型。在造模同时，各给药组灌胃给予相应的药物。试验第14d和21d，各组随机取5只大鼠，经腹腔取静脉血，测定SOD、GSH-Px活性和MDA、LD含量。结果模型对照组大鼠血清中SOD、GSH-Px活性明显降低俾〈0．05，P〈0．01），MDA、LD含量明显升高（P〈0．05）；给予抗衰老片后显著提高大鼠血清中SOD、GSH-Px活性和降低血清MDA、LD含量（P〈0．05，P〈0．01）。结论连续每日限制活动3h可使大鼠产生束缚应激，导致抗氧化能力降低，这可能与亚健康状态的形成有关。给予抗衰老片和六味地黄丸可明显提高血清中SOD、GSH—Px活性和降低MDA、LD含量。

Objective To observe the oxygen free radical change in sub-healthy rat induced by restraint stress and to explore the mechanism of Traditional Chinese Medicine alters the stress. Methods Fourty Male SD rats, weighted 320~360g, were divided randomly into 4 groups, which including normal control group, model control group, anti-aging tablets group and Liuweidihuang pills group, To simulate human physiological stress, the sub-healthy rat model was induced by restraint, meanwhile, the rats in anti-aging tablets group were administrated by anti-aging tablets and Liuweidihuang pills group were treated by Liuweidihuang pills separately. Fourteen and 21 days after experiment, 5 rats were selected randomly in each group to collect vein blood in order to test the activities of SOD,GSH-Px and the content MDA, LD. Results The activity of SOD and GSH-Px in blood serum in model rats lowered obviously （P〈0.05, P〈0.01） while the MDA, LD ascended significantly （P〈0.05）. The activity of SOD and GSH-Px in serum increased and the content of serum MDA, LD decreased significantly in anti-aging tablets group （P〈0.05, P〈0.01）. Conclusion The restraint stress for 3 hours everyday which could initiate the sub-healthy syndrome, and the antioxidation of model rat was decreased. The treatment of anti-aging tablets and Liuweidihuang pills could improve the sub-healthy state and the mechanism may be related to the effects on antioxidation.