Wnt/β-catenin信号通路在吡咯喹啉醌促雪旺细胞增殖中的作用研究

Effects of Wnt/β-catenin signal pathway in proliferation of Schwann cells promoted by pyrroloquinoline quinine

[目的]研究Wnt/β-catenin信号通路在吡咯喹啉醌促雪旺细胞增殖过程中的作用,探讨吡咯喹啉醌促进雪旺细胞增殖的可能的信号分子机制。[方法]体外培养纯化雪旺细胞,S-100免疫荧光鉴定;分别采用10nmol/L与100nmol/L吡咯喹啉醌作用雪旺细胞24h,观察雪旺细胞的形态学改变;分别用RT-PCR及Westernblot检测β-catenin在雪旺细胞经不同浓度吡咯喹啉醌作用72h后的表达情况。[结果]10nmol/L及100nmol/L吡咯喹啉醌均可促使雪旺细胞发生形态学上的变化,且吡咯喹啉醌的浓度为100nmol/L时对雪旺细胞的形态学影响更明显;RT-PCR及Westernblot结果均表明吡咯喹啉醌在1～1000nmol/L范围内可使雪旺细胞内β-catenin表达增加,当吡咯喹啉醌浓度为100nmol/L时其促进β-catenin表达的效果最强(P<0.05)。[结论]不同浓度的吡咯喹啉醌均可促使雪旺细胞发生形态学变化,使β-catenin表达增高;Wnt/β-catenin通路在吡咯喹啉醌促进雪旺细胞增殖过程中发挥作用。

[Objective]To investigate the effects of Wnt/β-catenin signal pathway on Schwann cells proliferation promoted by pyrroloquinoline quinine （PQQ） and its molecular mechanisms.[Method]Schwann cells were cultured and purified in vitro. The purity was identified by S-100. PQQ of 10 nmol/L and 100 nmol/L were added into culture medium for 24 hours,respectively. Then the morphological changes promoted by PQQ were observed by inverted microscope. The expression of β-catenin was detected by RT-PCR and Western blot in Schwann cells promoted by PQQ of different concentration for 72 hours.[Result]Morphological change was observed in Schwann cells treated by PQQ of 10 nmol/L and 100 nmol/L. The most obvious morphological changes took place in the Schwann cells treated by 100 nmol/L of PQQ,the RT-PCR and Western blot results showed that PQQ of 1-1000 nmol/L could up-regulate the expression of β-catenin,especially when Schwann cells was treated by PQQ of 100 nmol/L（P0.05）.[Conclusion]Different concentrations of PQQ could affect morphologic thange of Schwann cells and up-regulate β-catenin. These results suggest that Wnt/β-catenin signal pathway may be involved in Schwann cells proliferation promoted by PQQ.