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牙龈卟啉单胞菌表面相关物质对T淋巴细胞凋亡的影响 被引量：1

In vitro study of peripheral blood T lymphocyte activation and apoptosis induced by surface-associated material from Porphyromonas gingivalis
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摘要 目的研究牙龈卟啉单胞菌表面相关物质(SAM)对人外周血中T淋巴细胞活化及凋亡的作用。方法选取10名全身及牙周组织健康受试者,分离外周血中T淋巴细胞,在有/无SAM情况下培养0～96h,用荧光探针(AnnexinV-FITC、PI、CD69)进行标记,并进行流式细胞仪检测。结果 T淋巴细胞+SAM组AnnexinV+/PI-细胞百分数在48h、72h、96h分别为17.36±2.53,26.48±2.26,46.79±4.53。T淋巴细胞组AnnexinV+/PI-细胞百分数在48h、72h、96h分别为7.15±2.13,6.78±2.15,11.56±2.48,T淋巴细胞+SAM组与T淋巴细胞组之间存在明显差异(P<0.01)。CD69+T淋巴细胞+SAM组和CD69+T淋巴细胞组AnnexinV+/PI-细胞百分数除24h外的4个时间点上都无明显差异(P>0.05)。CD69+淋巴细胞+SAM组AnnexinV+/PI-细胞百分数在各个时间点上都明显高于T淋巴细胞+SAM组(P<0.01)。结论 SAM能够诱导人外周血中T淋巴细胞活化,并且能够通过活化促进其凋亡。 Objective To investigate the induction effects of surface-associated material from Porphyromonas gingivalis（SAM）on activation and apoptosis of the human peripheral blood T lymphocytes.Methods Peripheral blood T lymphocytes of healthy volunteers were isolated by Ficoll-Hypaque density gradient centrifugation.Then the cells were incubated with or without surface-associated material from Porphyromonas gingivalis over 0~96 h and the incubation was stopped at 0,24,48,72,96 hours,respectively.The cells were labeled with fluorochrome-conjugated monoclonal antibodies and probes（Annexin V-FITC,PI,CD69）and detected with flow cytometry.Results The percentages of Annexin V＋/PI-cells in the group of T lymphocytes with SAM were 17.36±2.53,26.48±2.26,46.79±4.53 at 48h,72h,96h,respectively;and those in the group of T lymphocytes without SAM were 7.15±2.13,6.78±2.15,11.56±2.48,respectively.The differences in percentages of Annexin V ＋/PI-cells between the two groups were significant（P0.01）.The percentages of Annexin V＋/PI-cells in the group of CD69＋T lymphocytes with SAM and the group without SAM were not significantly different（P0.05）at four time points except at 24 h.The percentages of Annexin V＋/PI-cells in the group of T lymphocytes with SAM were greater than those in the group of CD69＋T lymphocytes with SAM（P0.01）.Conclusion SAM could induce the activation of T lymphocytes and promote the apoptosis through activation.
作者 王惠宁 曹金芳 余杰
机构地区 温州医学院附属口腔医院口腔内科
出处 《口腔医学》 CAS 2010年第5期268-271,共4页 Stomatology
基金 温州市科技计划项目(Y20080020)
关键词 牙龈卟啉单胞菌表面相关物质 细胞凋亡 淋巴细胞 牙周病 surface-associated material from Porphyromonas gingivalis apoptosis T lymphocyte periodontitis
分类号 R780.2 [医药卫生—口腔医学]
  • 相关文献

参考文献13

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二级参考文献10

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共引文献3

同被引文献11

  • 1马净植,逄爱慧,李明.活动期与静止期牙周炎患牙龈沟液中IL-10和IFN-Y的含量测定[J].临床口腔医学杂志,2007,23(1):11-13. 被引量：12
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口腔医学
2010年 第5期

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