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氟达拉宾联合瘤苗抗小鼠RMA淋巴瘤的实验 被引量：1

Experiment of Anti-RMA Lymphoma of Mice by Fludarabine Peritoneal Injection Associated with Inactivated Lymphoma Vaccine
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摘要 目的研究注射用化疗药物氟达拉宾(Fludarabine)联合瘤苗治疗小鼠RMA淋巴瘤的作用与机制。方法以C57BL/6小鼠为研究对象,通过Western blot分析实验组与对照组小鼠脾脏组织中Foxp3基因的表达,并观察各组小鼠的肿瘤生长与生存期。结果氟达拉宾组与对照组相比,能够下调Foxp3基因的表达。氟达拉宾联合瘤苗能有效抑制肿瘤的生长速度,并提高荷瘤小鼠的生存期,与对照组相比差异有统计学意义(P<0.05)。结论氟达拉宾联合瘤苗能够增强小鼠机体抵抗肿瘤的能力,并能够延长小鼠生存期。 Objective To observe the anti-RMA lymphoma effect and mechanism by Fludarabine injection associated with inactivated lymphoma vaccine.Methods Use C57BL/6 mice as a target,Foxp3 genes in spleens were identified by Western blot,at the same time,the tumor growth and survival of the Fludarabine group and the control group were observed.Results Fludarabine could downregulate the expression of Foxp3 gene,and fludarabine associated with vaccine could not only inhibit the tumor growth but also improve survival of animals（P〈0.05）.Conclusion The experiment revealed that Fludarabine associated with vaccine can strengthen the ability to antagonize tumor,and prolog life span of tumor-bearing mice.
作者 李翔 高全立 买玲
机构地区 郑州大学基础医学院病理学与病理生理学系 河南省肿瘤医院血液科 河南省肿瘤医院科研外事科
出处 《肿瘤防治研究》 CAS CSCD 北大核心 2010年第5期519-521,共3页 Cancer Research on Prevention and Treatment
基金 河南省省属科研机构2006年度研究开发专项资金资助项目(0641130201)
关键词 氟达拉宾 瘤苗 FOXP3 免疫增强作用 Fludarabine Vaccine Foxp3 Immunologic enhancement
分类号 R733.4 [医药卫生—肿瘤]
  • 相关文献

参考文献7

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二级参考文献6

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同被引文献16

  • 1杨江华,张永祥,王芳,喻荣彬,苏川,孙南雄.人外周血CD4^+ CD25^+调节性T细胞的分离、鉴定和功能特征[J].细胞与分子免疫学杂志,2006,22(2):252-254. 被引量：18
  • 2李欣,崔永生,王炎,郑晓辉,李一.CD4^+ CD25^+调节性T细胞在Lewis肺癌移植鼠中的检测及临床意义[J].现代肿瘤医学,2007,15(4):454-457. 被引量：10
  • 3黄云胜,施志明.CD4^+CD25^+Tr细胞与非小细胞肺癌相关性研究[J].现代肿瘤医学,2007,15(7):928-929. 被引量：4
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  • 9Nair S, Boczkowski D, Fassnacht M, et al. Vaccination a- gainst the forkhead family transcription factor Foxp3 enhances tumor immunity[J]. Cancer Res, 2007, 67(1): 371- 380.
  • 10Sasada T, Kimura M, Yoshida Y, et aL CD4^+CD25^ + regula tory T cells in patients with gastrointestinal malignancies: pos sible involvenlent of regulatory T cells in disease progressian [J]. Cancer, 2003, 98(5): 1089-1099.

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肿瘤防治研究
2010年 第5期

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