ERCC1、P27^(kip1)、CyclinE在胃癌组织中的表达及其临床意义

Expression of ERCC1,P27^(kip1),CyclinE in Gastric Cancer and Its Clinical Significance

目的观察ERCC1、P27kip1、CyclinE在胃癌组织中的表达,探讨其在胃癌发生、发展中可能的作用及临床意义。方法采用免疫组织化学方法 (EliVisionTM二步法)检测手术切除的45例胃癌组织和10例正常胃组织中ERCC1、P27kip1、CyclinE的表达情况,并采用χ2检验和Pearson列联相关方法 ,分析ERCC1、P27kip1、CyclinE这三个指标与胃癌临床病理参数的关系及其三个指标的相关性。结果胃癌组织中ER-CC1、P27kip1、CyclinE阳性率分别为31.1%、35.6%、55.6%,ERCC1在胃癌组织中阳性率显著低于对照组(P<0.05);P27kip1在正常胃组织表达高于胃癌组、CyclinE在胃癌组表达高于正常对照组,两者差异均有统计学意义(P<0.05)。胃癌中ERCC1、P27kip1、CyclinE表达与肿瘤浸润深度、有无淋巴结转移、有无远处转移、临床病理分期有关(P<0.05),但与患者的性别、年龄、肿瘤大小、部位、分化程度无关(P>0.05);P27kip1、CyclinE之间存在负相关。结论 ERCC1、P27kip1的低表达、CyclinE的高表达与胃癌的发生、发展密切相关;ERCC1、P27kip1、CyclinE的表达水平可用于胃癌的诊断、鉴别及预后的判断;P27kip1与CyclinE蛋白在胃癌组织中表达成负相关,ERCC1与P27kip1、CyclinE蛋白的表达无相关性。

Objective To investigate the expression of excision repair cross complementing-1（ERCC1）,P27kip1 and CyclinE in gastric cancer and explore its role in development of gastric carcinoma and its clinical significance.Methods Expression levels of ERCC1,P27kip1 and CyclinE in 45 resected patients with gastric cancer and 10 normal tissues were detected by the method of immunohistochemistry（EliVisionTM）.We analyzed its correlation with gastric cancer in χ2 test and pearson analyze.Results The positive rates of ERCC1,P27kip1 and CyclinE protein expression was 31.1%,35.6% and 55.6% in gastric cancer,respectively.The positive rate of ERCC1 and P27kip1 in gastric cancer was lower than that in comparison group（P〈0.05）;The expression of CyclinE showed a significant difference from those of normal gastric mucosa（P〈0.05）.The expression level of ERCC1,P27kip1 and CyclinE was related to serosa invasion,lymphnode metastasis,distant metastasis and clinical histological grade（P〈0.05）.However,the expression of ERCC1,P27kip1 and CyclinE was not associated with gender,age,tumor size,position and differentiation.There was a highly negative correlation between P27kip1 and CyclinE expression（P〈0.05）.Conclusion The high expression of ERCC1,P27kip1 and the low expression of CyclinE were related to occurrence and development of gastric cancer.The detection of ERCC1,P27kip1 and CyclinE may be used for the diagnosis,identification and prognosis in patients with gastric cancer.There was a negative correlation between P27kip1 and CyclinE protein expression,but there was not correlation between expression level of ERCC1,P27kip1 and CyclinE in gastric cancer.