摘要
目的探讨survivin和CD34在胃癌发生、发展过程中的意义。方法采用PowerVision两步法检测survivin和CD34在胃癌和胃黏膜异型增生中的表达,用显微图像分析系统对survivin阳性表达进行图像分析,对CD34的表达进行微血管密度计数(MVD)。结果 survivin蛋白在正常胃黏膜组织中未见表达,在轻、中、重度异型增生和胃癌中均有不同程度的表达。积分吸光度在轻、中度异型增生与胃癌之间差异均有统计学意义(P<0.05),在重度异型增生与胃癌之间差异无统计学意义(P>0.05)。MVD在以上四组间均有统计学意义。survivin和CD34的表达与胃癌分化程度、淋巴结转移、胃癌浸润深度等病理特征有显著相关性(P<0.05);与患者的性别无统计学意义(P>0.05)。survivin的表达与MVD呈正相关。结论用吸光度参数定量分析胃癌免疫组织化学图像可以更客观地反映胃癌的发生、发展和预后。survivin蛋白可作为胃癌早期诊断的标志物。检测survivin和MVD有助于对胃癌的病理分级、恶性程度进行判定以及预后判断。
Objective To explore expression of survivin and CD34 in gastric carcinoma and dysplasia,and its significance in gastric carcinoma development.Methods Imunohistochemical PowerVision method was used to detect the expression of survivin and CD34 in gastric carcinoma and dysplasia.Quantitative image analysis was used to analyze the positive expression of survivin,micromodule density was usesd to indicate the expression of CD34.Results Survivin expression was negative in normal mucous membrane of gastric,but was positive in gastric carcinoma and dysplasia.Integrated optical density in gastric carcinoma was higher than that in dysplasia.There was no statistically significance between high dysplasia and gastric carcinoma.MVD was statistically difference among the four groups.The expression of survivin and MVD was associated with the differentiation degree,lymph node metastasis and infiltration degree of gastric cancer（P0.05）,but no relationship with sex（P0.05）.The expression of survivin was positive correlation with MVD.Conclusion Image analysis could reflect more objectively t0he produce,development,and prognosis of gastric carcinoma.survivin could be an index for early gastric carcinoma diagnosis and detecting survivin and MVD histological grading,tumor malignancy estimation and prognosis of gastric carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第5期544-546,550,共4页
Cancer Research on Prevention and Treatment
基金
陕西省教育厅自然科学专项基金资助项目(06JK318)
