细胞周期蛋白E1基因表达上调与胃癌发生的关系

Up-regulated expression of cyclin E1 is associated with gastric cancer development

目的在基因和蛋白水平检测胃癌组织中细胞周期蛋白E1(CCNE1)的表达,研究其与临床病理参数的相关性,探讨其与胃癌发生发展之间的关系。方法通过Oncomine(肿瘤微阵列数据库)分析CCNE1在胃癌中的表达。采用SYBR Green实时荧光定量PCR技术检测34例配对样本中CCNE1的mRNA的表达水平。采用组织芯片/组织微阵列技术结合免疫组化方法检测CCNE1在34例配对样本中的蛋白表达情况。结果胃癌原发灶CCNE1 mRNA表达水平显著高于癌旁正常组织(P=0.001),在胃癌组织中CCNE1 mRNA表达水平在Ⅰ+Ⅱ期和Ⅲ+Ⅳ期均呈现显著上调(P=0.042,P=0.016)。在胃癌原发组织中CCNE1蛋白阳性表达率[41.2%(14/34)]显著高于癌旁正常组织[0(0/34)]及胃炎组织[0(0/34)],P<0.01。CCNE1蛋白表达水平在不同分化程度、浸润深度及有无淋巴结转移肿瘤的表达不同,差异有统计学意义(P<0.05)。结论 CCNE1表达上调可能与胃癌的发生、发展密切相关,提示CCNE1的检测可能为胃癌的诊断和预后提供参考。

Objective To investigate the mRNA and protein expression of cyclin E1 （CCNE1） in gastric carcinoma tissues,and to evaluate its relationship with the development and progression of gastric carcinoma.Methods Oncomine （a cancer microarrays database） was used to analyze the expression of CCNE1 in gastric carcinoma.The mRNA expression of CCNE1 was detected in 34 paired carcinoma and adjacent normal tissues by SYBR Green real-time PCR.Meanwhile,tissue chip/tissue microarray （TMA） technique and immunohistochemistry method were adopted to detect the protein expression of CCNE1 in the 34 matched tissues.Results The CCNE1 mRNA expression was significantly higher in the primary carcinoma tissues than that in adjacent normal tissues （P=0.001）;the CCNE1 mRNA expression levels in stage Ⅰ ＋ Ⅱand stage Ⅲ ＋ Ⅳ gastric cancer tissues were both significantly up regulated （P=0.042,P=0.016）.The positive rate of CCNE1 protein was significantly higher in primary carcinoma tissues （41.2%,14 /34） than those in the tumor-adjacent normal tissues （0,0 /34） and gastritis tissues （0,0 /34,P=0.01）.CCNE1 protein expression was significantly different in gastric cancer tissues of different differentiation degrees,invasion depths,and lymph node metastasis statues （P〈0.05）.Conclusion Up-regulated expression of CCNE1 may contribute to the pathogenesis and progression of gastric carcinoma,and detection of CCNE1 may provide reference for diagnosis and prognosis of gastric carcinoma.