纤维素与明胶微载体微重力培养肝细胞的比较

Microgravity culture of hepatocytes on cellulose/gelatin macroporous microcarrier

目的探讨商品化的多孔微载体:cytopore 2和cultispher S何种更适合于微重力条件下培养CL-1细胞。方法采用RCCS旋转培养系统,在50ml水平微重力旋转培养CL-1细胞。分别对接种至cytopore 2和cultispher S的细胞进行动态形态学观察、细胞计数和功能学检测。结果CL-1细胞在两种载体上均可粘附生长,并于第9天达到生长高峰。细胞计数结果提示cultispher S的细胞密度可达到4×106个/ml。在第10、11、12天,cytopore 2组的上清中白蛋白浓度明显高于cultispher S组,有显著差异(P<0.05),在第10、11天,cytopore 2组的上清中尿素浓度明显高于cultispher S组,有显著差异(P<0.05)。结论Cytopore2培养的CL-1细胞计数低于Cultispher S组,但功能好于Cultispher S组。

Objective To compare macroporous microcarrier Cytopore 2 and Cultispher S for their effects in microgravity culture of CL-1 cells.Methods CL-1 cells were cultured on Cytopore 2 and Cultispher S respectively in a rotational cell culture system （RCCS） in a volume of 50 ml.Dynamic morphological observation and cell counting and functional test were carried out during the cell culture.Results The cells were capable of adhering to and proliferating on both of the microcarriers,reaching the growth peak on day 9 of cell culture with the maximum cell density of 4×106/ml on cultispher S.Albumin was significantly higher in the supernatant of Cytopore 2 than in that of Cultispher S on days 10,11,and 12（P0.05）,and the urea level in the supernatant of cytopore 2 was also significantly higher on days 10 and 11 （P0.05）.Conclusion The cells cultured on Cytopore 2,though with a smaller cell number,display better functions than those cultured on Cultispher S under RCCS conditions.