磁场对高脂高蛋白食谱诱发大鼠脂肪肝影响的机制研究

Magnetic fields ameliorates high-fat and high-protein diet-induced fatty liver in rats

目的研究磁场对高脂高蛋白食谱诱发脂肪肝影响的作用机理。方法采用进食高蛋白高脂肪饲料建立SD大鼠脂肪肝模型,磁场干预组选用低频旋转恒定磁场,建模10、14、18周动态检测各组血清和肝匀浆酶活性变化,同时用光镜和电镜观察肝组织在磁场作用下的变化情况。结果磁场干预组血清丙氨酸转氨酶、天门冬氨酸转氨酶含量降低,肝匀浆脂蛋白脂酶和肝脂肪酶、超氧化物歧化酶活性及丙二醛含量升高。光镜和电镜结果显示模型组肝细胞呈弥漫性脂肪变,有脂肪性坏死。磁场干预组肝细胞病理变化明显较轻。结论长期高脂高蛋白食谱作用诱发的脂肪肝在磁场作用下可以降低肝转氨酶,减少脂质过氧化,降低肝细胞脂肪变性的发生率。

Objective To study the mechanism of the effect of low-frequency rotary constant magnetic field on high-fat and high-protein diet-induced fatty liver in rats.Method Fatty liver model was established in SD rats by feeding on a high-fat and high-protein diet daily.The enzyme activity changes in the serum and liver homogenate were detected at 10,14,and 18 weeks,and the pathological changes of the liver were observed with optical and electron microscopy.Results In magnetic field intervention group,the concentration of alanine aminotransferase and aspartate transaminase were significantly decreased,and the activity of lipoprotein lipase,hepatic lipase,superoxide dismutase and the concentration of malondialdehyde in the liver homogenate were significantly increased.Under optical microscope and electron microscope,the rats in the model group showed diffusive adipose degeneration in the hepatic cells with large lipid droplets,which became large vacuoles after fat extraction,indicating fatty necrosis.In magnetic field intervention group,remarkably smaller lipid droplets and lessened hepatic cell adipose degeneration were observed.Conclusion Low-frequency rotary constant magnetic field has beneficial effect on fat metabolism,leading to reduced lipid peroxidation and structural recovery of the degenerated hepatic cells.