表达IDO的Kupffer细胞对小鼠移植皮片存活的影响

The Effect of Kupffer Cells Expressing Indoleamine 2,3-Dioxygenase on Mouse Allografted Skin

目的:探讨表达吲哚胺2,3-二氧化酶(IDO)的KC对同种异体小鼠移植皮片存活时间的影响及其机制。方法:构建BABL/c→C57BL/6的皮肤移植模型,分别于移植术后第2、7、14天输注KC,于移植术后第7天每组各取2只皮瓣行HE染色和TUNEL以检测淋巴细胞浸润和凋亡情况。Kaplan-Meier对数秩检验对各组进行生存分析。结果:输入表达IDO和FasL的KC能明显延长BABL/c→C57BL/6皮肤移植模型中皮肤移植物的存活时间,1-甲基色氨酸能阻断此效应。IFN-γ组皮瓣浸润淋巴细胞的凋亡率较高(P<0.05)。结论:表达IDO和FasL的KC在体内能明显延长同种异体小鼠皮片的存活时间,IDO在KC维持外周免疫耐受中发挥重要作用。

Objective：To investigate kupffer cells（KC） expressing indoleamine 2,3-dioxygenase（IDO） on the survival of grafted skin in mouse and its underlying mechanism.Methods：BABL/c skin was transplanted to C57BL/6. Donor KC were injected i.v.at days 2,7,14 before transplantatioa HE and TUNEL-AP were used to identify infiltrating cells and apoptotic cells in section of skin allografts from 7 days posttransplantation respectively.The survival rate of recipients among groups were analyzed by Log-rank test.Results：Injection of KC expressing IDO and FasL from BABL/c mice into C57BL/6 could prolong a skin graft survival from the donor,but 1-methyl-tryptophan could block the effect in vivo.The apoptosis rate of lymphocyte among skin graft in IFN-γgroup is more than other group（P0.05）.Conclusion：IDO and FasL-expressing KC from the donor of mouse can significantly prolong the skin graft survival.IDO may play an important role in KC to induce immune tolerance.