蛛网膜下腔出血致多器官功能障碍综合征大鼠延髓内脏带FOS蛋白的表达

FOS protein expression in the medullary visceral zone in rat model of multiple organ dysfunction syndrome induced by subarachnoid hemorrhage

目的观察蛛网膜下腔出血(SAH)致多器官功能障碍综合征(MODS)大鼠延髓内脏带(MVZ)FOS蛋白的表达规律,探讨MVZ在SAH致MODS中的可能调控机制。方法采用Willis环注血法建立大鼠SAH致MODS模型,免疫组织化学法检测MVZ内FOS蛋白的表达。结果(1)大鼠SAH后24～36h MVZ内FOS蛋白表达到高峰,且显著高于正常对照组和假手术组;(2)大鼠SAH后各时相点各脏器有不同程度的炎性损害,以24～36h为著;(3)SAH后全身炎症反应综合征和MODS发生率为100%和69.4%。结论SAH后各时相点各脏器的炎性改变与MVZ内FOS蛋白的表达规律相一致,提示MVZ参与了SAH后周围脏器功能的调控,是SAH致MODS的直接调控中枢之一。

Objective To investigate FOS protein expression in the medullary visceral zone （MVZ） of the rat model of subarachnoid hemorrhage （SAH） induced multiple organ dysfunction syndrome （MODS）,and to discuss the possible regulating mechanisms of MVZ in the development of MODS caused by SAH. Methods Rat model of SAH-induced MODS was prepared by injecting arterial blood into the Willis＇ circle. The expression of FOS protein in MVZ was analyzed immunohistochemically. Results The FOS protein expression in MVZ peaked at 24-36h after SAH,and was obviously higher than that in normal control group and sham-operation group. Most organs suffered inflammatory injuries at various degrees on each time point after SAH,which peaked at 24-36h. Incidence of systemic inflammatory response syndrome and MODS after SAH was 100% and 69.4% respectively. Conclusion FOS protein expression pattern parallels the histological changes of each organ following SAH. It indicates that MVZ may be one of the direct center regulating the development of SAH-induced MODS.